预处理和激素对草莓花药离体培养的影响  

Effect of Pretreatment and Hormone on Anther Culture of Strawberry Fragaria ananassa Var.Hongjia in Vitro

作　　者：陈纪鹏[1] 彭嗣亮 卢禹[1] 刘小林[1] 

机构地区：[1]宜春学院生命科学与资源环境学院/江西省植物生长发育调控重点实验室,江西宜春336000 [2]江西省邓家埠水稻原种场农业科学研究所,江西鹰潭335200

出　　处：《核农学报》2016年第11期2144-2150,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31460357);江西省科技支撑项目(20141BBF60010);江西省自然科学基金(20151BAB204026)

摘　　要：花药培养是进行植物脱毒和获取单倍体的有效方法。为提高草莓花药培养的再生效率,采用低温和黑暗的方式对草莓花药进行预处理,以不同种类和浓度的激素诱导诱导愈伤组织形成、不定芽形成与增殖。结果表明,培养前低温处理可促进愈伤组织形成,以4℃低温处理72h的效果最好。但花药黑暗预处理对愈伤组织形成没有作用。较高浓度的2,4-D(MS+1.0mg·L-16-BA+2.0mg·L-12,4-D)最适合诱导且促进花药愈伤组织形成,有助于下一步分化培养。而NAA对不定芽分化起着重要作用,诱导成功率达到10%以上,其中以MS+1.0mg·L-16-BA+0.2mg·L-1NAA诱导效果最好。在添加NAA的基础上再加入KT也能提高不定芽分化率。不定芽增殖过程不宜添加过高浓度的细胞分裂素和生长素,以MS+0.7mg·L-16-BA+0.1mg·L-1NAA诱导增殖效率最高,激素浓度过高时易出现玻璃化,现象,形成畸形苗。本研究建立了高效的草莓花药培养再生体系,为获得无病毒草莓苗和单倍体提供了条件。Anther culture is an effective method for plant detoxification and to get haploid. In order to raise the regeneration efficiency of anther culture of strawberry,pretreatment of strawberry（ Fragaria ananassa var. Hongjia）anther in low temperature and dark condition,calli and adventitious shoots induction of different auxine from anthers were studied as well. The results show that low temperature treatment under 4 ℃ condition for 72 h before culture can promote the formation of callus,whereas,it was no useful for induction of callus with treating anthers in darkness. High concentrations of 2,4-D（ MS ＋ 1. 0mg·L- 16-BA ＋ 2. 0mg·L- 12,4-D） contributed to inducing callus,special for valid ones. NAA played a more important role than other auxins in adventitious shoots induction,especially MS ＋ 1. 0mg·L- 16-BA ＋ 0. 2 mg·L- 1NAA had a high effiency and more than 10% calli can be induced to produce shoots.Cytokinin and auxin should not be added with too high concentration in adventitious shoot proliferation process,otherwise,shoots cannot develop to healthy plants,and the medium of MS containing 0. 7mg·L- 16-BA and 0. 1mg·L- 1NAA was the best. Virus free plant and haploid of strawberry can be obtained via anther regeneration in high frequency by theway.

关 键 词：草莓 花药培养 预处理 激素 快速繁殖 

分 类 号：S668.4[农业科学—果树学]