Vitek MS与Bruker MS对光滑念珠菌复合体鉴定能力评估  

Evaluation of the performance of Vitek MS and Bruker MS in identification of Candida glabrata species complex

作　　者：侯欣 张丽 王贺 肖盟 范欣 徐志鹏 于淑颖 康梅[2] 胡志东[3] 孙自镛[4] 万喆[5] 陈淑兰[6] 廖康[7] 褚云卓[8] 胡铁石[9] 倪语星[10] 邹桂玲[11] 徐英春 赵玉沛[12] 

机构地区：[1]中国医学科学院北京协和医院检验科中国医学科学院北京协和医学院研究生院,北京100730 [2]四川大学华西医院实验医学科,四川成都610041 [3]天津医科大学总医院医学检验科,天津300052 [4]华中科技大学同济医学院附属同济医院检验部,湖北武汉430030 [5]北京大学第一医院检验科,北京100034 [6]哈尔滨医科大学附属第一医院检验科,黑龙江哈尔滨150007 [7]中山大学附属第一医院检验科,广东广州510080 [8]中国医科大学附属第一医院,辽宁沈阳110001 [9]辽宁省人民医院检验科,辽宁沈阳110016 [10]上海交通大学医学院附属瑞金医院临床微生物与感染控制科,上海200025 [11]哈尔滨医科大学附属第四医院检验科,黑龙江哈尔滨150001 [12]中国医学科学院北京协和医院基本外科,北京100730

出　　处：《中华医院感染学杂志》2016年第18期4088-4091,共4页Chinese Journal of Nosocomiology

基　　金：卫生公益性行业科研专项(201402001);国家自然科学基金资助项目(81572057)

摘　　要：目的评估中国医院侵袭性真菌监测网（CHIF-NET）2010-2014年收集的光滑念珠菌复合体原始鉴定、Vitek MS与Bruker MS鉴定结果。方法收集CHIF-NET 2010-2014五年中12株Candida nivariensis和1株Candida bracarensis及来自CHIF-NET 2010-2014连续五年参加的11所医院的光滑念珠菌411株,所有菌株均经过分子生物学方法进行复核鉴定确认;以复核鉴定结果作为金标准,评估各医院原始常规方法鉴定结果,并评估Vitek MS和Bruker MS基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对光滑念珠菌复合体的鉴定性能。结果与分子生物学复核鉴定结果相比,光滑念珠菌的原始鉴定准确率为90.3%,9.7%的菌株被显色培养基、ID32C、API 20C或Vitek 2Compact错误鉴定为近平滑念珠菌、白色念珠菌等;常规方法无法鉴定C.nivariensise和C.bracarensis;Vitek MS和Bruker MS对光滑念珠菌的鉴定准确率分别为97.6%和100.0%;Vitek MS 2.0数据库中无C.nivariensise和C.bracarensis的参考谱图,故无法鉴定;Bruker Biotyper 3.1数据库中有这两种菌的参考谱图,但1株C.bracarensis无鉴定结果,12株C.nivariensise全部鉴定正确,鉴定分值为1.708~1.944。结论该研究率先在国内评估了Vitek MS与Bruker MS鉴定光滑念珠菌复合体的性能,两者对于光滑念珠菌的鉴定比较可靠;对于少见念珠菌菌种的鉴定,Bruker MS优于Vitek MS。OBJECTIVE To evaluate the results of original identification,Vitek MS and Bruker MS identification of Candida glabrata species complex collected from National China Hospital Invasive Fungal Surveillance Net（CHIF-NET）in 2010-2014.METHODS A total of one C.bracarensis isolate and twelve C.nivariensis isolates were collected from the CHIF-NET in 2010-2014,and 411 C.glabrataisolates were collected from eleven hospitals which participated in the CHIF-NET in five consecutive years from 2010 to 2014.All of the strains were identified by using molecular method,the review identification results were set as ＂gold-standard＂,the results of original identification of the conventional methods were evaluated,and the performances of Vitek MS and Bruker MS matrix assisted laser desorption ionization time of flight mass spectrometry（MALDI-TOF MS）in the identification of the C.glabrataspecies complex were observed.RESULTS As compared with the molecular identification,the accurate rate of original identification of C.glabrata was 90.3%,and 9.7% of the strains were mistakenly identified as Candida parapsilosis and Candida albicans by CHROMagar chromogenic media,ID 32 C,API 20 Cor Vitek 2Compact system.C.nivariensise and C.bracarensis could not be identified by using routine identification methods.The accurate rates of the Vitek MS and Bruker MS were 97.6% and 100.0%,respectively.There were no reference spectra for C.bracarensis and C.nivariensis in the Vitek MS 2.0database,so the two species could not be identified.There were reference spectra for the two species in the Bruker Biotyper 3.1database,however,one C.bracarensis isolate could not be identified and all of the 12 C.nivariensiseisolates were identified correctly,with the spectral score varying from 1.708 to 1.944.CONCLUSIONIt is for the first time to evaluate the performances of Vitek MS and Bruker MS in identification of the C.glabrata species complex in China and is concluded that the two methods are reliable for the identification of the C.glabrata.Bruker MS is

关 键 词：光滑念珠菌复合体 鉴定 基质辅助激光解吸电离飞行时间质谱 

分 类 号：R378.993[医药卫生—病原生物学]