产恩拉霉素链霉菌的诱变及其发酵条件的优化  

Inducing of Streptomyces Strain Producing Enduracidin and Optimization of Fermentation Conditions

作　　者：丁志雯[1] 胡永红[1] 杨文革[1] 吴刚[1] 顾鹏飞[1] 王春晓[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211816

出　　处：《贵州农业科学》2016年第7期72-76,共5页Guizhou Agricultural Sciences

基　　金：江苏省农业科技自主创新资金项目"一种新型高效动物专用多肽类生长调节剂恩拉霉素的发酵关键技术研究与产业化开发"[CX(12)3063]

摘　　要：为满足恩拉霉素工业化生产对高产菌株的需求,采用N+注入技术和单因素及正交试验方法对产恩拉霉素链霉菌(Streptomyces sp.)NJWGY3665进行诱变,并对目的菌株的最佳发酵条件进行优化。结果表明:随N+注入剂量的增加,菌株的存活率呈典型的马鞍型剂量-效应曲线。在最佳注射剂量120×1013ions/cm2条件下筛选到1株具有遗传稳定性的高产链霉菌突变菌株LD1;该菌株发酵最适培养基条件为蔗糖50g/L,酵母粉20g/L,NaCl 1.5g/L,FeSO_40.5g/L;最佳发酵条件为转速190r/min,温度28℃,pH 7,接种量8%。在此条件下,发酵液恩拉霉素浓度达11 860μg/mL。In order to meet the demand of industrialized production for high-yield strains producing enduracidin, the N＋ implantation technique was used for inducing the strain producing enduracidin Streptomyces sp. NJWGY3665 and the best fermentation condition of the high-yield strains was optimized through single factor and orthogonal experimental design. Results：With different doses of N＋ injected, the survival rate of the strain showed a typical saddle dose-response curve. Under the preferred dose 120 × 1013 ions/cm2, a high yield and good genetic stability strain LD1 was screened; The best medium of Streptomyces sp. NJWGY3665 were as follows： sucrose 50 g/L, yeast powder 20 g/L, NaCL 1. 5 g/L, FeSO4 0.5 g/L and the optimum fermentation conditions were： speed 190 r/min, temperature 28℃, pH 7, inoculum 8%, In these conditions, the enduracidin concentration reached 11 860 μg/mL in the fermented liquid

关 键 词：链霉菌 N+诱变 恩拉霉素 发酵条件优化 

分 类 号：S154.39[农业科学—土壤学] Q935[农业科学—农业基础科学]