生姜辛香物质合成途径关键基因ZoHMGR的克隆及表达  

Cloning and Expression of ZoHMGR(a Key Gene for Synthesis of Spicy Substance)in Zingiber officinale

作　　者：张绍彬[1] 唐海芹[1] 陈德碧[1] 兰建彬[2] 

机构地区：[1]重庆文理学院林学与生命科学学院,重庆402160 [2]重庆文理学院特色植物研究院,重庆402160

出　　处：《贵州农业科学》2016年第8期76-79,83,共5页Guizhou Agricultural Sciences

基　　金：重庆市教委项目"利用转录组测序挖掘合成生姜辛香和辛辣品质次生代谢物质关键基因"(KJ1401104);重庆文理学院校级项目"生姜辛香和辛辣品质相关的次生代谢物质测定与分析"(Z2013LX13);重庆文理学院校级人才引进项目"生姜辛香品质形成的分子调控机制"(R2012LS02)

摘　　要：为探明3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMGR)在生姜(Zingiber officinale Roscoe)倍半萜合成途径的调节作用,根据在竹根姜转录组数据库中HMGR不同转录本序列,经比对分析后利用RT-PCR技术克隆HMGR基因的全长cDNA序列,并对其进行生物信息学分析;再利用荧光定量PCR技术检测目的基因在竹根姜不同组织和叶组织在不同诱导条件下的表达特性,同时分析其生成的倍半萜物质。结果表明:克隆的竹根姜ZoHMGR基因编码562个氨基酸,其与姜科植物阳春砂的亲缘关系最近。ZoHMGR基因主要在根茎中表达,其次是叶,而茎和根中的表达量最低,符合生姜不同组织中倍半萜物质的含量特征;该基因同时受物理伤害和茉莉酸甲酯处理的诱导,分别在6h和12h达最高表达水平,同时有倍半萜类物质生成。The bioinformatics of full-length cDNA of ZoHMGR cloned by RT-PCR technology according to different transcript sequence of HMGR in the transcriptome database is analyzed.The expression characteristics of the target gene in different tissues and leaf of Zingiber officinale is detected by real-time PCR under different induction conditions and the synthetic sesquiterpene material is determined to explore the regulating effect of 3-Hydroxy-3-methylglutaryl-coenzyme A reductase（HMGR）in sesquiterpene synthesis of Zingiber officinale.Results：The cloned ZoHMGR has 562 amino acids,which is close to Amomum villosum.ZoHMGR expresses in rhizome mainly,followed by leaves.The expression quantity of ZoHMGRin stem and roots is minimum,which accords with the characteristics of sesquiterpenes content in different tissues of Zingiber officinale.The maximum expression level of ZoHMGRtreated by wound and MeJA induction is at 6hand 12 hrespectively and the sesquiterpenes material is synthesized at the same time.

关 键 词：生姜 倍半萜 HMGR基因 

分 类 号：S632.5[农业科学—蔬菜学]