猪苓微粉的膜分离及冷冻干燥效果  

Application of Membrane-separation and Freeze-drying Technology in Polysaccharide Extraction of Polyporus umbellatus

作　　者：陆宽[1] 汪凯莎[1] 刘建华 霍昕[1] 刘文炜[3] 冯发进 

机构地区：[1]贵州省生物技术研究开发基地,贵州贵阳550002 [2]贵州省中科院天然产物化学重点实验室,贵州贵阳550002 [3]贵阳中医学院第一附属医院,贵州贵阳550002

出　　处：《贵州农业科学》2016年第8期109-111,116,共4页Guizhou Agricultural Sciences

基　　金：贵州省社会发展科技攻关项目"猪苓;茯苓多糖制剂膜分离及生物活性研究"[黔科合SY字(2012)3080];贵州省开发类科研院所技术开发研究专项资金"生物基地技术转化中试平台建设"[黔科合成字(2013)5028];贵州省科技人才建设项目"贵州省药物固体制剂中试研究科技创新人才团队"[黔科合人才团队(2014)4011]

摘　　要：为有效利用并深度开发猪苓制品,采用膜分离技术将猪苓微粉提取液按膜孔径大小分离浓缩,对各截留段分离液采用冷冻干燥技术干燥,测定各截留段冻干粉得率和总糖含量。结果表明:猪苓微粉提取液按分离膜分子量大小被分成8段膜分离提取液,平均膜通量为6.93L/m^2·h。分子段UF100猪苓微粉冻干粉得率最高,为1.05%;UF5得率最低,仅0.07%。NF400与UF10的总糖含量最高,分别为91.12%和88.00%;NF200以下总糖含量最低,为34.15%。药材中UF100的冻干粉总糖含量占比最高,为0.76%;NF400次之,为0.35%。The extracting solution of P.umbellatus micro powder was separated and concentrated according to membrane pore size by the membrane-separation technology and the dry powder rate and total sugar content of different separation solutions from different retention level were determined to effectively utilize and deeply develop P.umbellatus product.Results：Eight extracting solutions of P.umbellatus micro powder are divided into according to separation membrane pore size and the average membrane flux is6.93L/m^2 h.The freeze-dried powder rate of P.umbellatus micro powder with UF100 molecular level is the highest（1.05%）and the freeze-dried powder rate of P.umbellatus micro powder with UF5 molecular level is the lowest（0.07%）.The total sugar content of P.umbellatus micro powder with NF400,UF10 and below NF200 molecular level is 91.12%,88.00%and 34.15%separately.The total sugar proportion in the freeze-dried powder with UF100 molecular level is the highest（0.76%）,followed by the freezedried powder with NF400（0.35%）.

关 键 词：猪苓微粉 提取 膜分离 冷冻干燥 

分 类 号：S38[农业科学—农艺学] R283.4[医药卫生—中药学]