三种生态环境下尼罗罗非鱼免疫因子的比较  

Comparison of Immune Factors of Nile Tilapia(Oreochromis niloticus)in Three Ecological Environments

作　　者：涂晨凌 陈辉辉[1] 巫立斌 于慧娟[1] 黄永春[1,2] 

机构地区：[1]集美大学水产学院,福建厦门361021 [2]福建省海洋渔业资源与生态环境重点实验室,福建厦门361021

出　　处：《水产科学》2016年第5期583-586,共4页Fisheries Science

基　　金：福建省山美水库库区生态系统调控与修复工程项目(2015);福建省教委项目(JA13174)

摘　　要：随机选取海水池塘（盐度28）和淡水池塘养殖及水库天然增殖的3种不同生态环境下、体质量为200～500g的尼罗罗非鱼为研究材料,采用生物化学方法测定3种生态环境尼罗罗非鱼血清中碱性磷酸酶、酸性磷酸酶、过氧化物酶、谷胱甘肽还原酶的活性和蛋白质量浓度。结果显示,3种生态环境中罗非鱼血清中碱性磷酸酶和酸性磷酸酶活力差异不显著（P〉0.05）,其余各组间罗非鱼血清中过氧化物酶和谷胱甘肽还原酶的活性及血清蛋白质量浓度差异显著（P〈0.05）,酶活力和蛋白质量浓度依次为：水库〉海水池塘〉淡水池塘。试验结果表明,水库天然增殖的尼罗罗非鱼具有较高的磷酸酶活性。The effects of three ecological environments on immune factors were evaluated in Nile tilapia （Oreochromis niloticus）. The Nile tilapia （body weight 200-500 g） were randomly selected as research materials from three ecological environment, namely seawater ponds（a salinity of 28）, freshwater ponds and reservoirs. The biochemical method was used to determine the activities of alkaline phosphatase （AKP）, acid phosphatase （ACP）, peroxidase （POD）, and glutathione reductase （GR） and protein concentrations in serum of Nile tilapia from three ecological environments. The results showed that there were no significant differences in the activities of AKP and ACP for individuals in the three ecological environments （P 〉 0.05）. The activities of POD and GR and protein concentrations were significant differences （P 〉 0.05）, with the order as reservoirs 〉 seawater ponds 〉 freshwater ponds. The Nile tilapia in res- ervoirs has high activity of phosphatase in serum.

关 键 词：尼罗罗非鱼 生态环境 免疫因子 

分 类 号：S917[农业科学—水产科学]