UPLC-MS/MS法测定牛奶中普鲁卡因青霉素G残留量  

Quantification of Penicillin G Procaine in Milk by UPLC-MS/MS

作　　者：陈雪 林婷 

机构地区：[1]上海德诺产品检测有限公司,上海200436

出　　处：《广州化工》2016年第18期149-150,共2页GuangZhou Chemical Industry

摘　　要：建立一种UPLC-MS/MS法（超高压液相色谱-串联质谱法）测定牛奶中普鲁卡因青霉素G的快速方法。采用正离子电离模式和MRM扫描模式。设置1.0,3.0,5.0μg/kg 3个加标水平,测定加标回收率。结果表明利用UPLC-MS/MS检测的普鲁卡因青霉素G在1.0~20.0 ng/m L的浓度范围内呈良好线性关系。3个加标水平的加标回收率为94.6~101.1%,相对标准偏差为1.3%~2.1%,检出限为1.0μg/kg。该方法快速,灵敏,准确,可满足普鲁卡因青霉素G残留量的测定。UPLC-MS/MS quantification performed with electrospray ionization in positive spiked concentration levels of 1.0 μg/kg, 3.0 of Penicillin G Procaine was built in milk. Qualitative analysis was mode （ESI） under multiple reaction monitoring （MRM） mode. There μg/kg, 5.0 /xg/kg were set for the determination of the recovery. The result showed that procaine quantification with UPLC-MS/MS had a linear relationship in the range of 1.0 - 20. 0 ng/mL. The average recoveries of the three spiked concentration levels were in the range of 94. 6% - 101.1% with RSD of 1.3% -2. 1% at 1.0-5.0 μg/kg （n=6）. The method is rapid, sensitive and accurate, which can meet the detection requirements of Penicillin G Procaine residues in milk.

关 键 词：超高压液相色谱-串联质谱法 普鲁卡因青霉素G 定量 牛奶 

分 类 号：S859.84[农业科学—临床兽医学]