HSF-1在HMGB1诱导的炎症反应中的作用  

Role of HSF-1 in inflammatory response induced by HMGB1

作　　者：尹朝奇[1] 贺全勇[1] 罗成群[1] 周建大[1] 陈佳[1] 李萍[1] 朱颉[1] 彭浩[1] 徐阳成[1] 陶如意[1] 

机构地区：[1]中南大学湘雅三医院烧伤整形科,湖南长沙410013

出　　处：《中国普通外科杂志》2016年第9期1291-1295,共5页China Journal of General Surgery

基　　金：湖南省卫生厅科研基金资助项目(B2012-030)

摘　　要：目的:探讨热休克转录因子1(HSF-1)在高迁移率族蛋白1(HMGB1)所诱导的炎症反应中的作用。方法:不同浓度HMGB1作用于RAW264.7细胞不同时间后,用ELISA法检测细胞上清液TNF-α水平;分别用免疫荧光法与Western blot法检测HMGB1作用后,RAW264.7细胞NF-κB核转移情况与HSF-1表达。观察干扰HSF-1表达后,HMGB1诱导RAW264.7细胞TNF-α表达的变化。结果:HMGB1作用后,RAW264.7细胞分别在4、12 h出现两次TNF-α分泌高峰,且TNF-α的释放量随HMGB1浓度的增加而增加。HMGB1作用后,RAW264.7细胞的NF-κB核转移明显增强,HSF-1表达明显增加(均P<0.05)。干扰HSF-1表达后,HMGB1诱导RAW264.7细胞产生的TNF-α量较未干扰的RAW264.7细胞明显增加(P<0.05)。结论:HMGB1能诱导RAW264.7细胞的炎症反应与HSF-1的表达,但HSF-1可能对HMGB1诱导的炎症反应有负反馈性抑制作用。Objective： To investigate the role of heat shock transcription factor 1 （HSF1） in inlfammatory response induced by high mobility group protein B1 （HMGB1）. Methods： hTe TNF-α contents in the supernatants of ARW264.7 cells atfer exposure to different concentrations of HMGB1 for different times were determined by ELISA assay. The NF-κB nuclear translocation and HSF-1 expression in ARW264.7 cells atfer HMGB1 treatment were examined by immunolfuorescence and Western blot, respectively. hTe change in TNF-α expression induced by HMGB1 in ARW264.7 cells atfer interference of HSF-1 expression was observed. Results： RAW264.7 cells presented two peaks of TNF-α release at 4 and 12 h respectively after HMGB1 treatment, and the TNF-α release level was increased with the elevation of HMGB1 concentration. hTe NF-κB nuclear translocation was signiifcantly enhanced and HSF-1 expression was signiifcantly increased in ARW264.7 cells after HMGB1 treatment （bothP〈0.05）. The TNF-α expression induced by HMGB1 in RAW264.7 cells＆amp;nbsp;atfer interference of HSF-1 expression was signiifcantly increased compared with ARW264.7 cells without HSF-1 interference （P〈0.05）. Conclusion： HMGB1 can induce inlfammatory response and HSF-1 expression in ARW264.7 cells, but HSF-1 may play a negative feedback inhibitory role in the HMGB1-induced inlfammation.

关 键 词：胰腺炎 急性坏死性 全身炎症反应综合征 高迁移率族蛋白质类 热休克转录因子1 

分 类 号：R657.5[医药卫生—外科学]