大豆疫霉病拮抗内生真菌的鉴定与筛选  

Identification and Screening of Endophytic Fungi against Phytophthora sojae

作　　者：刘永政[1] 边爽[1] 孙立杰[1] 宋佳宇[1] 董慧[1] 张玉琦[1] 赵静博[1] 肖佳雷[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《安徽农业科学》2016年第22期166-168,247,共4页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省自然基金项目(C2015008);黑龙江省哈尔滨市人才专项(2015RQQXJ019);国家青年基金项目(31101171);东北农业大学创新项目SPIT(201610224154)

摘　　要：[目的]筛选出拮抗致病大豆疫霉的真菌菌株,为深入研发生物菌剂提供依据。[方法]采用平板对峙法测定40株野生大豆真菌对疫霉菌的抑制作用。[结果]供试的40株菌株,不同菌株间菌落型态差异较大。从光滑程度分析,36份菌落表面不光滑,3份表面光滑并显浅黄色;从菌落颜色分析,正面以白色为主,其次是黄色和粉色;反面则以紫色为主,其次为黑色和绿色;从菌落生长型态分析,大多数菌落呈白色絮状突起或羊毛状菌丝;11株野生大豆内生真菌的抑菌率变化幅度为62%~81%,80%以上共1株Y4-S（Ⅰ）,75%~80%共2株,70%~75%共3株,其余5株均低于70%。抑菌率从大到小依次为Y4-S（Ⅰ）、Y5-r（Ⅱ）-1、Y6R-15-1、Y1-r-3和Y5R4、Y6S-26、Y5LB、Y5r-12、Y2-5（Ⅱ）、Y2-S、Y5R18-2-1。[结论]最强拮抗菌株Y4-S（Ⅰ）、Y5-r（Ⅱ）-1、Y6R-15-1显微型态相似,呈枝状,有隔,初步判断可能来源同一属,具体属种需要进一步判断。[Objective] Screening of antagonistic phytophthora pathogenic fungal strains provide the basis for further research and development of biological agents.[Method] Forty strains of fungus were tested.The inhibitory effect of wild soybean fungi on the wild soybean was deter-mined by the method of plate confrontation.[ Result] Among the tested 40 strains colony morphology are quite different from each other.On the analysis of smoothness, there are thirty-six colonies whose surface is not smooth, while three colonies appear smooth and pale yellow; On the analysis of colony colors, the front side is mainly white, followed by yellow and pink while, and the back side is mainly purple, followed by black and green;On the analysis of colony growth pattern, most of the colonies appear white flocculent protrusions or wool like hyphae.The inhibitory rates of 11 strains of wild soybean endophytic fungus are 62%-81%, with one strain of Y4-S （ I） more than 80%, two strains in 75%-80%, three strains in 70%-75%, and the remaining five strains are less than 70%.Inhibition rate in descending order is Y4-S（Ⅰ） , Y5-r（Ⅱ）-1,Y6R-15-1,Y1-r-3,Y5R4, Y6S-26, Y5LB, Y5r-12, Y2-5（Ⅱ）,Y2-S,Y5R18-2-1.[Conclusion] The strongest antagonistic effect of strains are Y4-S （Ⅰ）, Y5-r（Ⅱ）-1 and Y6R-15-1.They have similar microscopic form as dendritic, and they have separated.After a preliminary judgment, they may be a genus of unity, but their specific species require further judgment.

关 键 词：大豆疫霉病 野生大豆 内生真菌 拮抗菌株 

分 类 号：S565.1[农业科学—作物学]