检测牛奶中布鲁氏菌套式PCR方法的建立  

For detection of Brucella in milk nested PCR methods

作　　者：孙光野 张露[1] 王珊珊[1] 侯喜林[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《现代畜牧兽医》2016年第9期1-5,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：为了建立一种快速检测牛奶中布鲁氏菌的方法,本研究采用套式PCR的方法,通过对PCR条件的优化,改进布鲁氏菌提取DNA方法,经验证,最佳退火温度50.1℃、dNTP最佳浓度为0.2 mM、引物最佳浓度为0.4μM、Taq DNA聚合酶最佳浓度为0.1μL、本方法最小检测菌数为8个。说明用套式PCR的方法提高了检测牛奶中布鲁氏菌的敏感性。In order to establish a method for Brucella in milk rapid detection, this study nest- ed PCR method, by optimizing PCR conditions, improved Brucella DNA extraction method, proven, op- timal annealing temperature of 50.1℃, best dNTP concentration is 0.2 mM optimum primer concentration was 0.4 μM, Taq DNA polymerase, the optimal concentration was 0.1μL, the present meth- od is the minimum number of detected bacteria 8. The results show that by nested PCR method to im- prove the sensitivity of detection of Brucella in milk.

关 键 词：布鲁氏菌 套式PCR 奶样 

分 类 号：S852.619[农业科学—基础兽医学]