植物叶绿素含量不同提取方法的比较研究  

A Comparative Study on Different Extraction Methods for Plant Chlorophyll

作　　者：昌梦雨 魏晓楠[1] 王秋悦[1] 胡雨龙[1] 李承杭 唐延林[1] 

机构地区：[1]贵州大学理学院,贵阳550025

出　　处：《中国农学通报》2016年第27期177-180,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"烤烟理化参数的光谱监测机理与方法研究"(11164004);贵州大学"SRT"项目"植物叶绿素含量检测方法研究"

摘　　要：为探索不同提取液提取爬山虎叶绿素的效果,分别采用80%丙酮溶液、96%乙醇溶液、90%乙醇丙酮混合溶液、95%乙醇丙酮混合溶液4种提取溶液来提取五叶爬山虎叶片叶绿素。结果表明:叶绿素溶液吸收峰的峰值和不同提取液对植物叶绿素的提取能力随提取液的不同而不同,由大到小排序的顺序为95%丙酮乙醇溶液>90%丙酮乙醇溶液>80%丙酮>96%乙醇,相比于研磨法,用试剂浸泡法的叶绿素提取效率更高,这可为准确提取与检测植物叶绿素提供一定参考作用。To explore the effect of different extraction solutions on extracting boston ivy chlorophyll, the leaf chlorophyll of five-leaf boston ivy was extracted by four kinds of solutions such as 80% acetone solution, 96% ethanol solution, 90% ethanol acetone mixed solution and 95% ethanol acetone mixed solution, respectively. The results showed that the chlorophyll solution absorption peak values and the extraction ability of different extraction solutions were different with different plant chlorophyll extract solutions, from big to small was 95% ethanol acetone mixed solution〉 90% ethanol acetone mixed solution〉 80% acetone solution〉 96% ethanol solution. Compared with the grinding method, immersion method of extracting chlorophyll was more efficient. The study could provide certain references for accurate extraction and detection of plant chlorophyll.

关 键 词：爬山虎叶片 叶绿素含量 提取方法 

分 类 号：S127[农业科学—农业基础科学]