火炬姜种子无菌快速繁殖技术研究  

Study on Seeds Sterile Rapid Propagation of Phaeomeria magnifica(Roscoe)K.Shum

作　　者：吕德任 黄赛[1] 戚华沙[1] 王景飞[1] 符瑞侃 潘梅[1] 

机构地区：[1]海南省农业科学院热带园艺研究所,海南海口571100

出　　处：《黑龙江农业科学》2016年第9期19-24,共6页Heilongjiang Agricultural Sciences

基　　金：海南省科研院所技术开发研究专项资助项目(KYYS-2015-14)

摘　　要：为了加速火炬姜无菌培养,以火炬姜成熟种子作为外植体,通过比较MS无机盐浓度和植物生长调节剂种类及浓度配比、以及生根苗的移栽基质,建立火炬姜的组培快繁技术。结果表明:种子在培养基MS+6-BA2.0mg·L^(-1)中萌发;培养基MS+6-BA 3.0mg·L^(-1)+NAA 0.5mg·L^(-1)有利于丛生芽的生长发育,30d增殖系数为5.75;3/4MS+NAA 1.0mg·L^(-1)适宜诱导生根获得再生植株,生根率100%;生根苗移栽于椰糠中成活率98%,运用该组培快繁技术,可以高效繁殖火炬姜种苗。In order to accelerate the sterile culture of Phaeomeria magni f ica ,using the seeds as explants ,the techniques of tissue culture and rapid propagation were studied by comparing with different MS inorganic salt concentrations ,plant growth regulator types ,concentration ratio and transplanting substrates .The results showed that seeds sprouted buds in the medium MS ＋ 6‐BA 2 .0 mg·L‐1 ;MS＋ 6‐BA 3 .0 mg·L‐1 ＋ NAA 0 .5 mg·L‐1 could effectively induce fasciculate buds ,the propagation coefficient was 5 .75 every 30 days;the most optimum rooting medium was 3/4MS＋ NAA 1 .0 mg·L‐1 ,and the rooting rate was 100% .The rooting seedlings were effectively transplanted in coconut chaff ,the survival rate was 98% .Using the technology of tis‐sue culture and rapid propagation of Phaeomeria magni f ica could efficiently breeded seedlings .

关 键 词：火炬姜 种子 丛生芽 组织培养 快速繁殖 

分 类 号：S682.19[农业科学—观赏园艺]