Characterization of a collection of Chinese fir elite genotypes using sequence.related amplified polymorphism markers  

作　　者：Hongjing Duan Dehuo Hu Yun Li Huiquan Zheng 

机构地区：[1]National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China [2]Guangdong Provincial Key Laboratory of Bio-control for the Forest Disease and Pest, Guangdong Academy of Forestry,Guangzhou 510520, People's Republic of China

出　　处：《Journal of Forestry Research》2016年第5期1105-1110,共6页林业研究（英文版）

基　　金：supported by the National Natural Sciences Foundation of China(No.31200506);the Special Fund for Forest Scientific Research in the Public Welfare(No.201404127);the Special Plan for the Cultivation of High-level Talents of Guangdong(No.2014TQ01N140);the National‘863’Project of the 12th Five-Year Plan in China(2011AA100203)

摘　　要：Chinese fir [Cunninghamia lanceolata （Lamb.） Hook] is a major timber tree species in China. In the pre- sent study, sequence-related amplified polymorphism （SRAP） markers were used to evaluate the polymorphisms, diversity, and relationships in a collection of Chinese fir elite genotypes （n = 103） from the Lechang provenance, an endangered provenance. Thirty-five SRAP primer combinations produced 620 bands among the tested 103 Lechang provenance genotypes and 17 nonSLechang provenance genotypes; 577 （93.1%） of these were polymorphic, yielding an average of 16.5 polymorphic bands per primer combination. Of the SRAP primer combinations （n = 35）, I4 ones identified 〉70.0 % of the 120 genotypes. The Mel2-Eml9 combination had the strongest discriminatory ability with 93.3 % of the genotypes iden- tified. An UPGMA dendrogram further showed that most of the Lechang provenance genotypes （87.4 %） grouped together （Cluster A）. The Lechang genotypes had a con- siderable amount of genetic variation with genetic simi- larity from 0.40 to 0.81. The current work may facilitate the management of the endangered Lechang Chinese fir provenance.Chinese fir [Cunninghamia lanceolata （Lamb.） Hook] is a major timber tree species in China. In the pre- sent study, sequence-related amplified polymorphism （SRAP） markers were used to evaluate the polymorphisms, diversity, and relationships in a collection of Chinese fir elite genotypes （n = 103） from the Lechang provenance, an endangered provenance. Thirty-five SRAP primer combinations produced 620 bands among the tested 103 Lechang provenance genotypes and 17 nonSLechang provenance genotypes; 577 （93.1%） of these were polymorphic, yielding an average of 16.5 polymorphic bands per primer combination. Of the SRAP primer combinations （n = 35）, I4 ones identified 〉70.0 % of the 120 genotypes. The Mel2-Eml9 combination had the strongest discriminatory ability with 93.3 % of the genotypes iden- tified. An UPGMA dendrogram further showed that most of the Lechang provenance genotypes （87.4 %） grouped together （Cluster A）. The Lechang genotypes had a con- siderable amount of genetic variation with genetic simi- larity from 0.40 to 0.81. The current work may facilitate the management of the endangered Lechang Chinese fir provenance.

关 键 词：Chinese fir SRAP GERMPLASM Geneticvariation - Dendrogram 

分 类 号：S791.27[农业科学—林木遗传育种]