中医疗法对过敏性紫癜患者免疫功能的调节作用  

The effect of TCM therapy on immune function in patients with allergic purpura

作　　者：严峰[1] 许园园 王萍[3] 张艺凡[1] 吴姗姗[1] 

机构地区：[1]辽宁中医药大学附属医院临床检验中心,辽宁沈阳110032 [2]辽河油田第二职工医院检验科,辽宁盘锦124000 [3]辽宁中医药大学附属医院血液科,辽宁沈阳110032

出　　处：《检验医学与临床》2016年第18期2569-2572,共4页Laboratory Medicine and Clinic

基　　金：辽宁省省直医院改革重点临床科室诊疗能力建设项目青年项目(LNCCC-D52-2015)

摘　　要：目的探讨以疏风清热、活血化瘀立法治疗过敏性紫癜(HSP)前后,外周血白细胞总数、淋巴细胞数、淋巴细胞亚群、辅助性T细胞(Th)1/Th2及调节性T细胞的变化。方法使用血细胞分析仪检测36例住院患者血常规,统计其中的白细胞总数及淋巴细胞数。使用流式细胞仪检测36例住院患者外周血淋巴细胞亚群、Th1/Th2及调节性T细胞。结果 HSP患者治疗后(恢复期)与治疗前(急性期)相比较,白细胞数、淋巴细胞数、CD3^+、CD4^+、CD19^+降低(P<0.05),CD8^+、CD16^+CD56^+、Th1/Th2、CD4^+CD25^+升高(P<0.05);HSP患者治疗前与对照组相比较,白细胞数、淋巴细胞数、CD3^+、CD4^+、CD19^+明显升高(P<0.05),CD8^+、CD16^+CD56^+、Th1/Th2、CD4^+CD25^+降低(P<0.05);HSP患者治疗后与对照组相比较,白细胞数、淋巴细胞数、CD19^+升高(P<0.05);CD16^+降低(P<0.05);CD3^+、CD4^+、CD8^+、Th1/Th2、CD4^+CD25^+无差异(P>0.05)。结论以疏风清热、活血化瘀立法治疗HSP,能明显干预患者的外周血白细胞总数、淋巴细胞数、淋巴细胞亚群、Th1/Th2及调节性T细胞,对患者的免疫功能的调节作用明确,临床效果显著,极大地提高了临床诊疗能力。Objective To investigate the changes of peripheral blood leukocyte count ,lymphocyte count ,lymphocyte subsets , Th1/Th2 and regulatory T cells before and after the treatment of Henoch - Schnlein purpura （HSP） by dispelling wind and clearing away heat ,and promoting blood circulation and removing blood stasis .Methods The hematology analyzer was used to detect the blood routine in 36 inpatients ,in which leukocyte count and were performed statistics .The lymphocyte subsets ,Th1/Th2 and regu‐latory T cells were detected in these 36 inpatients by using flow cytometry .Results Compared with before treatment （acute phase） ,the leukocytes count ,lymphocytes count ,CD3 ＋ ,CD4 ＋ and CD19 ＋ after treatment （recovery phase） in the HSP patients were decreased （P 0 .05） .Conclusion Adopting the method of dispelling wind and clearing away heat ,and promoting blood circula‐tion and removing blood stasis for treating HSP can significantly interfere peripheral blood leukocytes count ,lymphocytes count , lymphocyte subsets ,Th1/Th2 and regulatory T cells of patients ,and has obvious effect for regulation the patient′ s immune func‐tion and remarkable clinical effect ,which greatly improves the ability of clinical diagnosis and treatment .

关 键 词：过敏性紫癜 淋巴细胞亚群 辅助性T细胞 抑制性T细胞 B淋巴细胞 自然杀伤细胞 TH1/TH2 调节性T细胞 

分 类 号：R47[医药卫生—护理学]