Reprimo基因3′非翻译区824位点多态性与肺癌发病的相关性研究  

The correlation between Reprimo 824 polymorphism and lung cancer

作　　者：陈贤华[1] 刘和艳[1] 詹前美[1] 徐玉婵 刘大鹰[2] 

机构地区：[1]柳州市柳铁中心医院检验科,广西柳州545007 [2]柳州市柳铁中心医院呼吸内科,广西柳州545007

出　　处：《检验医学与临床》2016年第18期2576-2578,2581,共4页Laboratory Medicine and Clinic

基　　金：广西壮族自治区卫生厅计划课题(Z2010138);广西壮族自治区柳州市科学技术局技术研究与开发计划课题(2011J0302031)

摘　　要：目的：探讨 Reprimo 基因3′非翻译区824位点 G ＞ C 位点单核苷酸多态性改变与肺癌发生的相关性。方法基因分型用 TaKaRa 公司的 MightyAmp DNA 聚合酶法，分别检测36例肺癌患者（癌症组）和23例非癌症患者（对照组）的 Reprimo基因3′非翻译区824位点 G ＞ C 分布，运用χ2检验、Logistic 回归分析和 Armitage′s 趋势检验分析基因多态性、患者性别、吸烟与否、年龄与肺癌发生的相关性。结果两组标本经哈迪‐温伯格平衡检测，对照组在 Reprimo 基因3′非翻译区824位点 G ＞ C 基因型 GG 、GC 、CC 观测频数分别为21、1、1，理论频数分别为20．01、2．80、0．10，差异无统计学意义（P＝0．067）；癌症组的 GG 、GC 、CC 基因型观测频数分别为33、1、2，理论频数分别为31．17、4．65、0．17，差异有统计学意义（P＝0．003）。 Logistic 回归分析显示，年龄、基因多态性、患者性别和吸烟与否在肺癌发生中均不是危险因素（P＞0．05）；经 Armitage′s 趋势检验显示，Reprimo 基因3′非翻译区824位点多态性与肺癌发生之间无相关性（χ2 ＝0．00，P＝0．946）。结论 Reprimo 基因3′非翻译区824位点 G ＞ C 单核苷酸位点多态性与肺癌发生不具有相关性。Objective To investigate the relationship between Reprimo gene 3′‐untranslated region 824 G 〉 C polymorphism and lung cancer .Methods Genotyping was performed by using MightyAmp DNA Polymerase ,36 lung cancer patients（cancer group） and 23 lung cancer patients（control group） were genotyped .The results of investigation were analyzed with χ2 test ,Logistic regres‐sion and Armitage′s trend test .Results According to Hardy‐Weinberg equilibrium examination ,the observed frequencies of GG , GC and CC in Reprimo 824 G 〉 C genotype of control group were 21 ,1 and 1 ,respectively ,while the theoretical frequency were 20 .01 ,2 .80 and 0 .10 respectively（no statistically significant difference was observed ,P= 0 .067） .The observed frequencies of GG , GC and CC in Reprimo 824 G 〉 C genotype of cancer group were 33 ,1 and 2 ,respectively ,while the theoretical frequency were 31 .17 ,4 .65 and 0 .17 ,respectively（no statistically significant difference was observed ,P= 0 .067） .Tested by using Logistic regres‐sion ,gene polymorphism ,age ,smoking status and lung cancer（P〉 0 .05） .Armitage′s trend test revealed no statistically significant correlation between Reprimo 824 G 〉 C polymorphism and susceptibility to lung cancer （χ2 = 0 .00 ,P = 0 .946） .Conclusion This study indicates that there is not an association between the Reprimo gene 3′‐untranslated region 824 G 〉 C polymorphism and lung cancer .

关 键 词：肺癌 Reprimo 基因 多态性 哈迪-温伯格平衡 

分 类 号：R457.7[医药卫生—治疗学]