祁连山不同海拔低温原油降解菌群的分布特性研究  

Elevation-distribution characteristics of the crude oil degrading bacterium in the Qilian Mountains

作　　者：杨蕊琪 薛林贵[1] 常思静[2] 章高森[2] 陈拓[2] 刘光琇[2] 张勇[3] 高海宁[3] 

机构地区：[1]兰州交通大学化学与生物工程学院甘肃省极端环境微生物资源与工程重点实验室,甘肃兰州730070 [2]甘肃省极端环境微生物资源与工程重点实验室/中国科学院寒区旱区环境与工程研究所沙漠与沙漠化重点实验室,甘肃兰州730030 [3]河西学院农业与生物技术学院,甘肃张掖734000

出　　处：《冰川冻土》2016年第3期785-793,共9页Journal of Glaciology and Geocryology

基　　金：国家国际科技合作专项项目(2014DFA30330);甘肃省国际科技合作项目(1304WCGA173);国家自然科学基金项目(31260090)资助

摘　　要：以祁连山南麓中端野牛沟土壤为研究对象,研究了不同海拔下低温原油降解细菌群落的分布特征.结果显示：研究区域具有原油利用潜力的低温降解菌数量为0.3×10^5～1.4×10^5CFU·g^-1,随海拔的升高,菌群明显减少;同时通过16S r RNA基因序列分析共发现4个门、7个属、25种细菌,其中假单胞菌属（Pseudomonas）和节杆菌属（Arthrobacter）是优势属,并且位于海拔3 597 m处的菌株原油利用潜力均较高,而变形菌门的不动杆菌属（Acinetobacter）利用原油生长的潜力最高.In this study,the distribution characteristics of low -temperature crude oil degrading bacterial population in soil from Yeniugou in the south side of the Qilian Mountains was investigated. The results showed that the quantities of degrading bacterium in this region were between 0.3×10^5～1.4×10^～5CFU·g^-1,which also decreased with altitude. Meanwhile,through 16 S r RNA gene sequence analysis,it was detected that there were four phylum,seven genera and 25 species;among them,Pseudomonas and Arthrobacter were predominant genera. It was also found that the strains isolated from the altitude of 3 579 m had relatively higher utilization potential and the strains belonging to the genus Acinetobacter had the highest utilization potential for crude-oil.

关 键 词：祁连山 海拔 低温 原油降解菌 

分 类 号：Q938.1[生物学—微生物学]