PiggyBac转座子在肝脏中的活性研究  

The activity of PiggyBac transposon in liver

作　　者：刘华华[1] 缪辉[1] 葛梦芸 吴传芳[1] 

机构地区：[1]四川大学生命科学学院功能基因组实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2016年第5期1130-1134,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(31300674)

摘　　要：本实验运用高压尾静脉注射的方法将PB转座系统导入小鼠肝脏,研究其在肝脏中的转座活性,系统中PB转座子携带表达红色荧光蛋白的基因以指示转座的发生.注射10个月后取出小鼠肝脏,体视荧光显微镜观察肝脏是否有红色荧光,并通过基因组PCR、splinkerette PCR分析PB转座子在肝脏中的插入情况.实验结果表明,PB转座子在肝脏内发生高效转座,检测的68个PB插入位点中有34个位于基因序列,使得PB系统可以作为有效的基因诱变工具来研究基因功能.In this study, PB binary cotransfection system was directly delivered into liver via hydrody- namic injection using RFP （Red Fluorescence Protein） as the indicator of PB transposition. Ten months later of injection, tests were carried out to detect the red fluorescence in liver. Then, genomic PCR and splinkerette PCR were performed to analyze the insertion sites of PB transposon. The results showed that PB transposon has an efficient transposition in liver and 34/68 insertion sites were located in gene sequence.

关 键 词：PiggyBac(PB)转座子 高压尾静脉注射 Splinkerette PCR 插入突变 

分 类 号：Q789[生物学—分子生物学]