补肾、活血复方对骨质疏松症模型大鼠VEGF表达的影响  

Effect of the nourishing kidney and promoting blood circulation compound on VEGF expression in rat osteoporosis model

作　　者：孙宁[1] 邓洋洋[1] 孙鑫[1] 尚德阳[1] 林庶茹[1] 郑洪新[1] SUN Ning DENG Yangyang SUN Xin SHANG Deyang LIN Shuru ZHENG Hongxin(Liaoning University of Chinese Medicine, Shenyang 110847, China)

机构地区：[1]辽宁中医药大学,沈阳110847

出　　处：《中国骨质疏松杂志》2016年第9期1096-1100,共5页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金青年科学基金项目(81302879)

摘　　要：目的比较补肾、活血复方对骨质疏松症模型大鼠VEGF表达的影响,探讨补肾、活血复方治疗骨质疏松症的分子机制。方法以去卵巢骨质疏松症模型大鼠为实验研究对象,不同实验药物灌胃12周后,双能X线法测定骨密度,ELISA法测定骨、肾及血清中VEGF含量,定量PCR法测定骨、肾中VEGF mRNA相对表达量。结果骨质疏松症模型大鼠骨密度、VEGF蛋白及mRNA表达量显著降低,运用补肾、活血中药复方干预后,各组大鼠骨密度、VEGF蛋白含量及mRNA表达量显著升高。结论肾虚血瘀骨代谢失常与VEGF表达降低有关,补肾、活血复方通过调节VEGF表达水平,起到治疗骨质疏松症的作用。Objective To compare the effect of the nourishing kidney and promoting blood circulation compound on VEGF expression of rat osteoporosis model,and to investigate the molecular mechanism of the nourishing kidney and promoting blood circulation compound in the treatment of osteoporosis.Methods Ovariectomized osteoporosis rat model was used for experimental objective.The rats were given the medicines by intragastric administration for 12 weeks.Bone mineral density was measured using dual-energy X-ray absorptiometry.The content of VEGF in the bone,kidney,and the serum were determined using ELISA method.The relative expression of VEGF mRNAs in the bone and the kidney were determined using qPCR method.Results The expressions of VEGF protein and mRNA in osteoporosis model rats decreased significantly.After the intervention with the nourishing kidney and promoting blood circulation compound,the expressions of VEGF protein and mRNA of each group increased significantly.Conclusion The disorder of bone metabolism due to renal deficiency and blood stasis is related to low expression of VEGF.The nourishing kidney and promoting blood circulation compound can treat osteoporosis through regulation of the expression of VEGF.

关 键 词：中医中药 补肾 活血 骨质疏松症 VEGF 

分 类 号：R285.5[医药卫生—中药学]