一株重组鸭源H5N2亚型禽流感病毒分子进化分析  

Molecular Phylogenetic Analysis of a Highly Pathogenic H5N2 Avian Influenza Virus Isolated from Duck

作　　者：姚艳丰 何斌 邵志勇 杨文海 刘武 陈夏冰 叶胜强 陈洁 

机构地区：[1]武汉市农业科学技术研究院畜牧兽医科学研究所,武汉430208

出　　处：《病毒学报》2016年第5期590-596,共7页Chinese Journal of Virology

基　　金：武汉市农科院创新项目(项目号:CX201609-04;CX201607);国家自然科学基金(项目号:31400787)

摘　　要：2016年对武汉地区家禽市场进行常规流感监测,分离鉴定到1株H5N2亚型禽流感病毒。本研究对该株病毒进行了全基因组测序,分子特征和遗传进化分析。结果显示该株病毒的HA基因属于Clade2.3.4.4分支,HA蛋白的裂解位点处具有多个连续碱性氨基酸,具备高致病性禽流感病毒的典型分子特征。序列比对分析显示该株病毒的各基因节段分别与H5不同亚型的禽流感病毒具有较高的相似性,推测该分离株为重组病毒。继续开展对家禽市场H5亚类流感病毒的分子流行病学调查,对研究高致病性流感病毒的变异和进化,以及对禽流感的综合防控有着重要的意义。In 2016,routine influenza virus surveillance was conducted in the live poultry markets of Wuhan,Hubei Province.An H5N2 subtype avian influenza virus（AIV）was isolated from ducks in Wuhan.The entire genome of this virus isolate was sequenced,and molecular phylogenetic analysis performed.The results indicated that the HA gene belonged to clade 2.3.4.4and contained multiple basic amino acids at the cleavage site,which is characteristic of highly pathogenic AIV.Sequence alignment revealed that the isolate shared a high degree of homology with different H5 subtype AIVs isolated from waterfowl in southern China in recent years.This isolate was likely a natural reassortant from different subtype AIVs.This study provides epidemiological evidence of influenza evolution.Continuation of molecular epidemiology studies of H5 subtype influenza viruses in live poultry markets is important for understanding their role in the variation and evolution of highly pathogenic AIVs and their potential hazardous effects on human health.Furthermore,this information is important for strengthening comprehensive AIV surveillance and control measures.

关 键 词：禽流感 H5N2亚型 鸭 分子进化分析 

分 类 号：S852.62[农业科学—基础兽医学]