基于六种有效成分的大孔树脂纯化葛根总异黄酮工艺研究  

Study on Purification Process of Six Kinds of Effective Components of Isoflavonoids from Puerariae lobatae by Macroporous Adsorption Resin

作　　者：贺志龙[1] 时晓媞 顾健[2] 汪涓[3] 黄福开[3] 谭睿[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031 [2]西南民族大学民族药物研究院,四川成都610041 [3]中国藏学研究中心北京藏医院,北京100029

出　　处：《现代中药研究与实践》2016年第4期45-50,共6页Research and Practice on Chinese Medicines

基　　金：国家"重大新药创制"科技重大专项:葛黄有效组分防治2型糖尿病候选药物研究(2013ZX09103-002-014);国家自然科学基金面上项目:三个藏药经典方早中晚组合给药协同治疗脑缺血机制探讨(81274184);国家自然科学基金:从对炎症通路的影响探讨藏药波棱瓜子及其有效成分抗免疫肝损伤的作用及机制(81674168);南京大学国家重点实验室开放基金:藏药波棱瓜子抗肝炎成分研究(KF-GN-201404);国家财政专项:藏医心脑血管临床研究与药物研发(1981420400011)

摘　　要：目的研究大孔树脂分离、纯化葛根中总异黄酮的工艺条件。方法以3’-羟基葛根素、3’-甲氧基葛根素、葛根素、大豆苷元-8-C-芹菜糖-(1→6)葡萄糖苷、大豆苷、大豆苷元6种成分的吸附量和解吸率为评价指标,考察9种不同类型树脂。并对优选出的树脂纯化葛根总异黄酮的工艺条件及参数进行考察。结果 HPD600大孔树脂吸附、解吸效果最好,上柱药液浓度为75 mg/ml,吸附上样流速1BV/h,径高比1:10,除杂溶剂为水,除杂体积5 BV,除杂流速1 BV/h,洗脱溶剂为50%乙醇,洗脱体积6BV,洗脱流速2 BV/h。结论葛根中6种异黄酮成分精制后含量保留均在80%以上,总异黄酮纯度在60%以上,表明优选的AB-8大孔树脂纯化葛根总异黄酮工艺是可行的。Objective To investigate the process of isolation and purification of total isoflavonoids from Puerariae lobatae by macroporous adsorption resin. Methods The adsorption amount and desorption ratio of the six kinds of isoflavonoids were chosen as indexes for refining to evaluated nine kinds of macroporous adsorption resins.Also the process conditions and parameters of total isoflavonoids from Puerariae lobatae by optimized macroporous adsorption resin were researched. Results The consequence is type of HPD600 had the best dynamic adsorptivity and separability to the total isoflavonoids from Puerariae lobatae when the content in the aqueous extract was 75 mg per 1 ml equivalent to raw material, the ratio of diameter and height was 1：10, velocity of adsorption was 1 BV/h, solvent of removing impurities was water, volume of removing impurities was 5 BV, velocity of removing impurities was 1 BV/h, solvent of elution was 50% of ethanol, volume of elution was 6 BV, velocity of elution was 2 BV/h. Conclusion The content of six kind isoflavonoids in Puerariae lobatae were retained more than 80%, besides the purity of total isoflavonoids was over 60% after isolation and purification by macroporous adsorption resin. It is concluded that the optimized process is feasible.

关 键 词：葛根 总异黄酮 大孔树脂 精制工艺 

分 类 号：R284.2[医药卫生—中药学]