绞股蓝正丁醇提取物对快速老化小鼠Aβ蛋白的影响  

Effect of n-butanol extract from Gynostemma pentaphyllum on the expression of amyloid β protein in senescence accelerated mice

作　　者：吴燕春[1] 包传红[1] 阎莉[1] 钟振国[1] 颜其双 

机构地区：[1]广西中医药大学,广西南宁530001 [2]广西壮族自治区食品药品检验所,广西南宁530021

出　　处：《中成药》2016年第9期1898-1902,共5页Chinese Traditional Patent Medicine

基　　金：广西自然科学基金(2010GXNSFD013050);广西高等学校高水平创新团队及卓越学者计划资助项目(桂教人[2014]7);广西高校重点实验室(壮医方药基础与应用研究重点实验室)项目(桂教科研[2014]6)

摘　　要：目的探讨壮药绞股蓝Gynostemma pentaphyllum正丁醇提取物对快速老化SAMP8小鼠淀粉样蛋白(Aβ蛋白)的影响。方法 50只快速老化SAMP8小鼠随机分为模型组、石杉碱甲组[0.15 mg/(kg·d)]和绞股蓝正丁醇提取物高、中、低剂量组[500、250、125 mg/(kg·d)],10只正常老化SAMR1小鼠为正常对照组。灌胃给药8周后,取小鼠血清和脑匀浆,以ELISA法检测其Aβ蛋白的水平,RT-PCR和免疫组织化学法检测App基因和APP蛋白的表达。结果与模型组比较,绞股蓝正丁醇提取物给药组小鼠脑组织中的APP基因表达和蛋白表达明显下调,血清和脑组织中的Aβ蛋白水平显著降低。结论绞股蓝正丁醇提取物可通过抑制APP蛋白的生成,使Aβ蛋白的表达减少,从而起到防治阿尔茨海默症(AD)的作用。AIM To explore the effect of n-butanol extract from Gynostemma pentaphyllum,a native herb of the Zhuang nationality,on the expression of Aβ protein in SAMP8 senescence-accelerated mice. METHODS Fifty SAMP8 senescence-accelerated mice were randomly divided into five groups,model,positive drug [Huperzine-A,0. 15 mg /（ kg·d） ],high-,medium- and low-dose of n-butanol extract from Gynostemma pentaphyllum groups [500,250,125 mg /（ kg·d） ]. Furthermore,ten SAMR1 normal aging mice were used as normal control.After 8-week intragastric administration,the serum and brain homogenate of mice were collected to detect the Aβprotein level with enzyme linked immunosorbent assay（ ELISA）,App gene expression by real-time polymerase chain reaction（ RT-PCR）,and APP protein expression using immunohistochemistry. RESULTS Compared with the model group,the lower expressions of App gene and APP protein in brain tissue of mice could be observed in each group treated with n-butanol extract from Gynostemma pentaphyllum. This result was accompanied by significantly reduced Aβ protein levels in serum and brain tissue. CONCLUSION The n-butanol extract from Gynostemma pentaphyllum can restrain APP protein formation,and reduce Aβ protein expression,which plays a role in the prevention and treatment of Alzheimer＇s disease.

关 键 词：绞股蓝正丁醇提取物 快速老化小鼠 淀粉样前体蛋白(APP蛋白) 淀粉样蛋白(Aβ蛋白) 

分 类 号：R285.5[医药卫生—中药学]