耐冬山茶抗寒基因CjCor1的全长克隆与表达分析  

Cloning and Expression Analysis of CjCor1 Gene cDNA from Camellia japonica (Nai Dong)

作　　者：杨凯[1,2] 孙迎坤[2] 王奎玲[2] 刘庆华[2] 高捍东[1] 

机构地区：[1]南京林业大学,南京210037 [2]青岛农业大学,青岛266109

出　　处：《植物研究》2016年第5期753-759,共7页Bulletin of Botanical Research

基　　金：山东省高等学校科技计划项目(J13LE16);"十二五"农村领域国家科技计划课题(2012BAD01B0703)~~

摘　　要：COR基因是植物冷驯化过程中重要的功能基因。为研究Cor基因在耐冬山茶(Camellia japonica)抗寒方面的功能,采取同源克隆的方法,从自然低温条件下的耐冬山茶叶片中分离到了617 bp的耐冬山茶同源COR基因全长序列,命名为Cj Cor1。该序列全长完整,含有600 bp的开放阅读框。氨基酸序列分析显示,Cj Cor1基因编码的199个氨基酸蛋白质,含有冷驯化蛋白家族WCOR413 Superfamily特有的保守域结构,在同属物种中的保守性极高。荧光定量PCR结果表明,在低温(0℃以下时)条件下,相比南方山茶原种和常见栽培品种,Cj Cor1基因在耐冬山茶中具有较高表达量,且在耐寒性高的品种中的表达量也较高。COR genes play important roles in plant cold acclimation process. In order to study the Cor gene function of Camellia japonica to severe cold, adopting homologous cloning method, a 617 bp full-length eDNA sequence was cloned from leaves of C. japonica （ Nai Dong） under the natural low temperature condition, named CjCorl. The total length sequence is complete, and contains an opening reading frame of 600 bp. Amino acids sequence alignment shows that CjCorl gene encodes a protein of 199 amino acids, containing specific conservative domain structure of WCOR413 in cold acclimatization protein superfamily, and highly conservative property in species belonged to same genus. By real-time PCR analysis, comparing with southern Camellia species and common varieties, CjCorl genes had higher expression in C. japonica （Nai Dong ） under the condition of low temperature（ below 0℃ ）, and also had higher expression in higher cold resistance varieties.

关 键 词：耐冬山茶 CjCor1基因 序列分析 基因表达 

分 类 号：S685.14[农业科学—观赏园艺]