极度濒危植物巧家五针松基因组微卫星特征分析  

Characterization of Microsatellites in Genome of Pinus squamata,a Critically Endangered Species in the World

作　　者：阮桢媛 王兵益[2] 欧阳志勤[3] 廖礼彬[2] 苏腾伟 乔璐 

机构地区：[1]云南林业职业技术学院,昆明650224 [2]中国林业科学研究院资源昆虫研究所,昆明650224 [3]云南省环境科学研究院,昆明650034

出　　处：《植物研究》2016年第5期775-781,共7页Bulletin of Botanical Research

基　　金：云南省教育厅科学研究基金项目(2014Y569);云南省教育厅科学研究基金项目(2014Y571);云南省生物多样性保护专项资金资助项目(2013-004);云南林业职业技术学院博士基金项目(KY(BS)201402)~~

摘　　要：巧家五针松是世界极度濒危植物,对其SSR引物的开发有助于其遗传学研究以及物种的保护。本研究通过Illumina高通量测序技术获得巧家五针松全基因组序列,并以MISA软件查找得到2 651个微卫星序列,其中单核苷酸重复最多,可能预示了其悠久的进化历史。不同重复类型中,A/T含量显著高于G/C;在不同长度重复单元中,二核苷酸重复微卫星长度变异程度最高;各重复类型微卫星长度与微卫星出现的频率成反比。获得的微卫星序列能够满足巧家五针松的种群遗传学研究,而且反映了该物种的偏好性及对应的潜在功能,并且对该物种的保护提供了资料。Pinus squamata is a critically endangered species in the world, therefore, developing simple sequence repeat （SSR） primers will facilitate the species genetic research and conservation. The complete genome sequence of P. squamaia was determined through Illumina genome analyzer, and 2 651 microsatellites were discovered by MISA software. Single nucleotide repeat number occupied the highest proportion of these microsatellites which may indicate a long evolution history of P. squamata. Among different repeat types, A/T contents was significantly higher than G/C contents. In terms of distinct length repeat units, the length of dinucleotide repeat microsatellite varies with the highest degree. The length of SSRs with different repeat type was negatively correlated with frequency of occurrences. The available microsatellite sequences can satisfy P. squamaia population genetics, and reveal the species bias and corresponding potential functions.

关 键 词：巧家五针松 基因组 微卫星 

分 类 号：S791.24[农业科学—林木遗传育种]