东北地区非鹅膏属真菌中鹅膏肽类毒素的分布  

Distribution characteristics of amatoxins in fungi excluding Amanita

作　　者：任荆蕾 图力古尔[1] 包海鹰[1] 

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,吉林长春130118

出　　处：《菌物学报》2016年第9期1080-1098,共19页Mycosystema

基　　金：国家自然科学基金(31093440,31493010,31493011);教育部“长江学者和创新团队发展计划”(IRT1134)~~

摘　　要：本文采用高效液相色谱(HPLC)法对采自中国东北地区的95份非鹅膏属真菌样本进行鹅膏肽类毒素(α-amanitin、β-amanitin、phalloidin)检测。为获得更准确的实验结果,对57份阳性反应样品利用UPLC-MS/MS进一步测定,值得注意的是,测定结果中褶纹丝盖伞Inocybe leiocephala确认同时含有以上3种毒素。本研究通过检测鹅膏肽类毒素在非鹅膏属真菌中的分布,对误食中毒事件的发生起到一定程度的预防和警示作用,另一方面筛选出含有鹅膏肽类毒素的新型毒菌资源,为毒菌资源的开发利用提供基础资料。The α-amanitin, β-amanitin and phalloidin were detected in 95 fungus samples from different genus excluding Amanita from Northeast China by using HPLC method. For the purpose of obtaining more accurate results, UPLC-MS/MS were used for further determination of 57 positive samples. It is particularly worth mentioning that Inocybe leiocephala is proved to be a fungus containing simultaneously α-amanitin, β-amanitin and phalloidin. The results of this study could provide a reference for preventing and warning poisoning, and screening and exploiting new toxin resources from poisonous mushrooms.

关 键 词：鹅膏肽类毒素 HPLC法 UPLC-MS/MS法 

分 类 号：Q949.32[生物学—植物学]