2013～2015年浙江省宁波市麻疹流行病学和基因型特征分析  

Epidemiological Characteristics and Genotypes of Measles Cases in Ningbo City of Zhenjiang Province during 2013-2015

作　　者：顾文珍[1] 倪红霞[1] 马瑞[1] 刘昱慧[1] 傅小红[1] 张姝[1] 焦素黎[1] 

机构地区：[1]宁波市疾病预防控制中心,浙江宁波315010

出　　处：《中国疫苗和免疫》2016年第4期385-389,共5页Chinese Journal of Vaccines and Immunization

基　　金：宁波市科技创新团队项目(编号:2012B82018);浙江省医学重点学科(编号:07-013)

摘　　要：目的了解2013-2015年宁波市麻疹流行病学和基因型特征。方法统计分析2013-2015年宁波市麻疹监测数据，收集各县（市、区）病例咽试子标本进行麻疹病毒分离，经逆转录-聚合酶链反应（RT-PER）对核蛋白N基因羧基末端进行扩增和测序，测序结果与GeneBank中各基因型参考株比对分析。结果2013～2015年宁波市共报告麻疹509例，发病年龄以〈8月龄和〉20岁为主。共分离到麻疹野病毒60株，其中49株为H1a基因型，它们之间的N基因羧基末端的核苷酸和氨基酸同源性分别为93．63％-100％和94．44％-100％，与疫苗株S191的核苷酸和氨基酸同源性分别为88．66％～92．71％和85．48％～89．31％；其余11株为B3基因型，它们之间的N基因羧基末端的核苷酸和氨基酸同源性分别为98．2％-100％和96．6％-100％；与疫苗株S191的核苷酸和氨基酸同源性分别为91．1％～93．2％和86．4％～88．9％；与中国湖南分离株同源性分别97．29％～99．11％和97．29％～99．33％；与中国香港分离株同源性分别为99．78％～97．97％和98％～100％。结论2013～2015年宁波市麻疹疫情比较稳定，HIa为优势基因型，输入性B3基因型可小范围流行，疫苗株S191对B3基因型有一定保护作用。Objective To evaluate the epidemiological characteristics and genotype distribution of measles cases in Ningbo during 2013-2015. Methods Measles case data were described with epidemiological methods. Measles viruses were isolated from specimens from suspected measles cases. The nucleoprotein （N） genes were amplified by reverse transcription-polymerase chain reaction （RT-PCR）, sequenced, and compared with reference strains from Genebank. Results In total, 509 measles cases were reported in Ningbo during 2013-2015, and most cases were among infants 〈 8 months old or among adults 〉 20 years old. Sixty measles viruses were isolated from these cases; 49 were genotype H1 a, and 11 were gen- otype B3. Homology of the N genes in nucleotide （nt） and amino acid （aa） between Hla strains were 93.63%-100% and 94. 44%-100% , respectively, with a respective homology of 88.66%-92.71% and 85.48% -89.31% compared with 5191 vaccine strain. The homology of N genes in nt and aa between B3 strains were 98.2%-100% and 96.6%-100%, with a respective homology of 91.1%-93.2% and 86.4%-88.9% compared with S191 vaccine strain, a respective homology of 97.29%-99.11% and 97.29%-99. 33% compared with strains isolated from Hunan province, or a respective homology of 99.78%-97.97% and 98%-100% compared with strains isolated from Hong Kong. Conclusions Mea-sles incidences were stable in Ningbo from 2013 to 2015. Genotype Hl a was the predominate strain of measles virus. Imported genotype B3 might cause small epidemics, and it ean be partly pro- teeted by the S191 vaccine strain.

关 键 词：麻疹病毒 基因型 序列分析 核蛋白 

分 类 号：R373.1[医药卫生—病原生物学]