SOX5转录因子在人类精液中的表达  

Expression of transcription factor SOX5 in human semen

作　　者：邹沙沙[1] 杨施[1] 肖倩[1] 孙璨[1] 卢克敏[1] 施文博[1] 刘勇[1] 邱进[1] 杜艳芝[1] 陈向锋[1] 

机构地区：[1]上海交通大学医学院附属仁济医院生殖医学中心 上海市人类精子库 上海市辅助生殖与优生重点实验室,200135

出　　处：《中华医学杂志》2016年第36期2876-2879,共4页National Medical Journal of China

基　　金：国家自然科学基金青年基金（81501243）;上海市卫计委科研课题（201440089）

摘　　要：目的分析SOX5基因在人类精液中所表达的转录变体类型及其表达差异，为探讨SOX5在睾丸生精过程中的作用提供一定依据。方法随机抽样法收集2015年9月上海市人类精子库志愿供精者的61份精液样本和3份血液样本（血液样本作为对照）。主要采用琼脂糖凝胶电泳和实时荧光定量PCR（RT-qPCR）等方法检测SOX5基因在人类精液中的转录变体类型及其表达情况。结果在精液中可检测到不同类型的SOX5转录变体，即长链形式（L）-SOX5和短链形式（S）-SOX5。其中，S-SOX5特异表达于精液中，而在对照血液中未检测到表达。L-SOX5和S-SOX5主要存在于精浆中，精子本身的表达量较低。同时，定量分析发现在精液中L-SOX5的转录变体表达量（0.280±0.232）高于S-SOX5的表达（0.147±0.103），但差异无统计学意义（P＝0.132）。结论SOX5在精液中存在不同类型的转录变体，不同类型的SOX5转录变体在精液中的表达量也不同。Objective To study and analyze the transcript and expression pattern of SOX5 in human semen, thus to provide evidence for exploring the role of transcription factor SOX5 in the process of spermatogenesis in testis. Methods A total of 61 semen samples and 3 blood samples （ as controls ） collected from healthy volunteer donors at Shanghai Human Sperm Bank in September 2015 were randomly selected. Expression of SOX5 in the semen samples was detected and quantified using agarose gel electrophoresis and real time-quantitative PCR （RT-qPCR） analysis. Results In the human semen samples, SOX5 was found in two different transcript forms： long transcript （L-SOX5） and short transcript （S-SOXS）. Moreover, the S-SOX5 was specifically expressed in human semen, not detected in the control blood samples. L-SOX5 and S-SOX5 were primarily found in seminal plasma, with low expression level in sperms. The results of RT-qPCR analysis showed obviously higher mRNA expression levels of L-SOX5 than S-SOX5 in semen （0. 280 ± 0. 232 vs 0. 147 ± 0. 103 ）, however, not significantly（ P = 0. 132）. Conclusion SOX5 is expressed as different transcripts in human semen, and different transcripts may play different roles in the process of spermatogenesis in testis.

关 键 词：人类 精液 SOX5转录因子 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]