大鼠睾丸组织中抑制素B表达下调与生精功能障碍的关系  

Relationship between decreased expression of inhibin B and spermatogenesis dysfunction in rats testis

作　　者：张杰 金芃芃[2] 方恺[1] 伊庆同[1] 唐美芳[3] 陈楚红[1] 郭建华[4] 龚旻[1] 

机构地区：[1]复旦大学附属浦东医院泌尿外科,上海201399 [2]复旦大学附属浦东医院医学科研与创新中心,上海201399 [3]复旦大学附属浦东医院检验科,上海201399 [4]上海交通大学附属第九人民医院泌尿外科

出　　处：《中华医学杂志》2016年第36期2880-2884,共5页National Medical Journal of China

基　　金：上海市浦东新区卫生和计划生育委员会科技发展专项基金（PW2013A-21）;浦东新区卫生系统重点学科建设项目（PWZx2014-04）

摘　　要：目的探讨精索静脉曲张对大鼠睾丸组织表达抑制素B的影响及其与生精功能障碍之间的关系。方法SPF级SD雄性大鼠20只，按随机数字表法分为对照组和实验组。实验组采取左肾静脉部分结扎法制造精索静脉曲张的模型，对照组只做精索内静脉分离，不做结扎，其余同实验组。通过组织化学评价各组大鼠的生精功能及分析抑制素B表达情况，反转录（RT）－PCR检测抑制素B、Fas及Fas配体（FasL）mRNA的表达情况，Western印迹检测抑制素B蛋白的表达。结果大鼠左侧睾丸生精功能比较：实验组Johnsen评分小于对照组[（9.79±0.05）比（9.97±0.02）分，P＝0.023]；实验组生精上皮厚度低于对照组[（48.35±0.99）μm比（57.58±1.98）μm，P＝0.000]；实验组的左侧附睾精子计数少于右侧[（933±161）×10^6个/（ml·g）比（1 552±184）×10^6个/（ml·g），P＝0.017]。Western印迹灰度分析显示实验组抑制素B的表达（0.407±0.053）低于对照组（0.608±0.076）（P＝0.038）。免疫组化检测亦显示实验组抑制素B的表达（0.161±0.004）低于对照组（0.183±0.005）（P＝0.008）。RT－PCR检测实验组的抑制素B mRNA表达量比对照组下降（0.522±0.050比1.10^6±0.210，P＝0.003）；实验组的Fas mRNA表达量比对照组下降（P＝0.019）；实验组FasL mRNA表达量比对照组升高（P＝0.015）。结论精索静脉曲张可引起大鼠睾丸组织抑制素B表达下降和生精功能障碍，抑制素B表达下调与生精功能障碍有密切关系，抑制素B在精索静脉曲张引起不育的机制中发挥重要作用。Objective To explore the effects of varicocele on the expressions of inhibin B in rat testes and the relationship between expression of inhibin B and spermatogenesis dysfunction. Methods Twenty specific-pathogen-free Sprague-Dawley （SD） male rats were randomly divided into two groups with random number table： 4-week control group （C4） and 4-week experimental group （V4）. Experimental varicocele was created by partial ligation of left renal vein in the V4 group ; vein isolation without ligation was performed in the C4 group. Spermatogenetic function in the two groups were assessed. The expression of inhibin B in the rat testis was analyzed by immunohistochemistry, the expressions of inhibin B, Fas and Fas ligand （FasL）mRNA measured by reverse transcription-polymerase chain reaction （RT-PCR）, and the inhibin B protein expression by Western blot. Results In comparison of spermatogenetic function in left rat testes, the Johnsen scores in the V4 group were significantly lower than those in the C4 group （9.79 ± 0. 05 vs 9. 97 ± 0. 02,P = 0. 023 ） ,the seminiferous epithelium in the V4 group was significantly thinner than that in the C4 group [ （48.35 ±0. 99） μm vs （57.58 ± 1.98） μm,P =0. 000] , and the number of sperms in the left epididymis was significantly lower than in the right one in the V4 group [ （933 ± 161 ）×10^6/（ ml·g） vs （ 1 552 ± 184）×10^6/（ ml·g） ,P =0. 017]. Both Western blot and immunohistochemical assay showed that the expression of inhibin B in the rat testes was significantly lower in the V4 group than in the C4 group （0. 407 ±0. 053 vs 0. 608±0. 076,P =0. 038;0. 161±0. 004 vs 0. 183 ±0. 005,P =0. 008）. RT-PCR also detected reduced expression of inhibin B mRNA in the V4 group compared with the CA group （0. 522 ± 0. 050 vs 1. 106 ±0. 210,P =0. 003. Compared to the C4 group, the expression of Fas mRNA in the V4 group was significantly lower, while the expression of FasL mRNA in the V4 group was significantly higher （ P = 0. 019,

关 键 词：精索静脉曲张 抑制素类 精子发生 睾丸 

分 类 号：R698[医药卫生—泌尿科学]