在线GPC-GC-MS/MS法直接检测烟叶中的农药残留  

Determination of pesticide residues in tobacco by on-line gel permeation chromatography-gas chromatography/tandem mass spectrometry

作　　者：罗彦波[1] 李翔宇[1] 朱风鹏[1] 张洪非[1] 姜兴益[1] 李雪[1] 庞永强[1] LUO Yanbo LI Xiangyu ZHU Fengpeng ZHANG Hongfei JIANG Xingyi LI Xue PANG Yongqiang(China National Tobacco Quality Supervision ＆ Test Center, Zhengzhou 450001, China)

机构地区：[1]国家烟草质量监督检验中心

出　　处：《烟草科技》2016年第9期42-49,共8页Tobacco Science & Technology

摘　　要：为了测定烟叶中的农药残留,通过优化收集时间和质谱检测参数,将烟叶样品提取后直接用在线凝胶渗透色谱-气相色谱-串联质谱法（GPC-GC-MS/MS）测定,建立了烟叶中31种农药残留的检测方法。结果表明：①在优化的条件下,目标分析物的检出限在1.95～212 ng/L之间,决定系数均大于0.99,日内和日间相对标准偏差分别不大于6.3%和10.2%,实际样品的加标回收率为82.2%～121.5%。②该方法成功应用于实际烟叶样品的检测,且检测结果与标准方法相吻合。In order to analyze the pesticide residues in tobacco leaves, tobacco extract was directly analyzed by on-line gel permeation chromatography-gas chromatography-tandem mass spectrometry. This method was able to quantify the residue levels of 31 pesticides, and the optimal determination conditions were obtained via optimizing gel permeation chromatography collection time and multiple reaction monitoring parameters, which could influence the determination results. The results showed that： 1） Under the optimized conditions, the limits of detection of the method for the target analytes ranged from 1.95 to 212 ng/L, the determination coefficients （R2） were more than 0.99 for all the target analytes, the intra- and inter-day relative standard deviations were no more than 6.3% and 10.2%, respectively; and the recoveries of standard were from 82.2% to 121.5% for test samples. 2） The method was successfully applied to the analysis of pesticide residues in tobacco samples, the test results well agreed with those of existing standard methods.

关 键 词：烟叶 农药残留 在线凝胶渗透色谱-气相色谱-串联质谱(在线GPC-GC-MS/MS) 直接检测 

分 类 号：S481.8[农业科学—农药学] TS411.1[农业科学—植物保护]