胸腺素β4介导牵张成骨区骨生成的作用及机制研究  

Bone formation mediated by local administration of thymosin 154 in distraction osteogenesis

作　　者：陈红浩[1] 贾亚超[1] 康庆林[1] 梁川[1] 柴益民[1] 

机构地区：[1]上海交通大学附属第六人民医院,200233

出　　处：《中华创伤骨科杂志》2016年第9期791-797,共7页Chinese Journal of Orthopaedic Trauma

基　　金：国家自然科学基金面上项目（81272000）

摘　　要：目的探讨胸腺素B4对大鼠牵张成骨区骨生成的作用及机制。方法将60只SD大鼠随机分为A、B、C3组。分别于左股骨行截骨术外固定建立牵张成骨模型，牵张期结束时A组不予处理，B组于牵张成骨区注射磷酸盐缓冲液，C组于牵张成骨区注射等量胸腺素B4。术后第22、29、43天分别处死大鼠，取材行X线、Micr-CT、HE染色、免疫组化染色检测牵张成骨区的骨生成情况，逆转录聚合酶链式反应（RT-PCR）检测相关基因的转录水平。结果X线检测显示C组术后第22、29、43天成骨优于A、B组。Micro-CT检测显示C组术后第22、29、43天取材标本骨体积、骨矿化密度、骨体积分数高于A、B组，C组术后第43天骨表面体积分数小于A、B组，差异均有统计学意义（P〈0．05）。HE染色显示C组局部毛细血管密度明显高于A、B组。免疫组化染色显示c组成骨区毛细血管密度增多以及成骨细胞在截骨断端募集，均高于A、B组。RT—PCR检测C组第22天内皮型一氧化氮合酶（eNOS）、OsterixmRNA的转录水平高于A、B组，差异均有统计学意义（P〈0．05）。结论在牵张成骨区局部应用外源性胸腺素B4可促进成骨区新生血管形成与骨生成，可能与牵张成骨区eNOS、Osterix的表达增高有关。Objective To explore the effect and mechanism of local administration of thymosin 134 （Tβ4） on bone regeneration in a distraction osteogenesis model of rat femur. Methods Sixty Sprague-Dawley rats were randomly divided into groups A, B and C in this study. A distraction osteogenesis model was established in the left femur after osteotomy. At the end of distraction period, the bone regeneration area in group A was subjected to no treatment, that in group B .to injection of phosphate buffer saline （PBS）, and that in group C to injection of Tβ4. On days 22, 29 and 43 postoperatively, the rats from each group were randomly sacrificed and processed for observation of bone regeneration in the distraction osteogenesis area using radiography, Micro-CT, histology and immunohistochemical staining. RT-PCR was used to detect the ex- pression of related genes as well. Results Radiography revealed that the bone regeneration in group C was superior to that in groups A and B on days 22, 29 and 43 postoperatively. Micro-CT examination showed significantly increased bone volume （BV）, bone mineral density （BMD） and ratio of bone volume to tissue volume （BV/TV） in group C on days 22, 29 and 43 postoperatively, and significantly decreased ratio of bone surface area to bone volume on postoperative day 43 in comparison with groups A and B （ P 〈 0. 05） . HE staining indicated that local capillary density was significantly higher in group C than in groups A and B after local administration of Tβ4 in the distraction osteogenesis. Immunohistochemical staining showed that the capillary density and osteobtasts in group C were higher than in groups A and B. RT-PCR results revealed significantly higher expression of eNOS and Osterix mRNA in the local callus in group C on postoperative day 22 than in groups A and group B （ P 〈 0. 05） . Conclusion Local administration of thymosin β4 may promote bone formation, which is probably related to the increased expression of eNOS and Osterix.

关 键 词：胸腺素 骨延长术 骨生成 牵张 外固定器 

分 类 号：R68[医药卫生—骨科学]