富含Gln小麦水解蛋白对DSS诱导小鼠结肠炎的作用研究  

Effect of Wheat Protein Hydrolysate with Rich Glutamine on Mucosal Barrier of DSS Induced Colitis Mice

作　　者：何林玲[1] 王学东[1] 李丽[2] 李彪[3] 

机构地区：[1]武汉轻工大学食品科学与工程学院,武汉430023 [2]武昌工学院,武汉430065 [3]安琪酵母股份有限公司,宜昌443000

出　　处：《中国粮油学报》2016年第9期7-12,共6页Journal of the Chinese Cereals and Oils Association

基　　金：武汉轻工大学研究生教育创新计划(2013cx016)

摘　　要：探讨了富含谷氨酰胺(Gln)小麦水解蛋白对葡聚糖硫酸钠(DSS)诱导小鼠结肠炎的影响。将24只小鼠分为正常对照组(Ⅰ组)、DSS诱导模型组(Ⅱ组)和营养液组(Ⅲ组)。观察了临床表现及结肠黏膜的病理组织变化,检测了结肠组织的抗氧化活性,荧光定量PCR测定了结肠组织中炎症因子的表达。结果表明,营养液处理后,减轻了由结肠炎造成的结肠黏膜的病理损伤,结肠组织中的IFN-γ和IL-6的表达较模型组低,而IL-10和TGF-β的表达差异不显著,表明富含Gln的小麦水解蛋白可以促进促炎/抑炎因子的相对平衡,从而缓解肠道炎症,是一种潜在的抗氧化和抗炎资源。In order to explore the influence of wheat protein hydrolysate rich in glutamine （Gln） on dextran sodi- um sul^te （DSS） induced colitis in mice, using DSS induced mice to establish the colitis model, after the completion of colitis model hydrolyzed wheat protein rich in nutrient solution Gln was irrigated for continuous 7 d, and mice would be put to death. Through observing the clinical manifestations and pathological tissue changes of colonic mucosa,colon tis- sue myeloperoxidase （ MPO）, superoxide dismutase （SOD）, reduced glutathione （ GSH）, and malondialdehyde （MDA） of activity were detected ; using the fluorescent quantitative PCR to determine the expression of cell inflammatory factors in the colon tissues. The results showed that MPO activity and MDA content in colon tissue were reduced after affected by wheat protein hydrolysate nutrient solution, and SOD activity and GSH content were increased,reducing colonic mucosa pathological damage caused by colitis. The expression of inflammatory factors also shows that wheat protein hydrolysate nutrient solution rich in Gln had repairing functions on colon injuries of DSS induced mice.

关 键 词：谷氨酰胺 DSS 小鼠结肠炎 炎症因子 

分 类 号：S-3[农业科学]