复方葛根片对老龄大鼠肝细胞再生与凋亡的影响  

Effects of Compound Puerarin Tablet on the Regeneration and Apoptosis of Liver Cells in Aged Rats

作　　者：王飞飞[1] 李允江[1] 杨文超[1] 路其康 于燕莉[2] 

机构地区：[1]山东中医药大学药学院,济南250355 [2]济南军区总医院药剂科,济南250031

出　　处：《中国药房》2016年第28期3942-3944,共3页China Pharmacy

摘　　要：目的:研究复方葛根片对老龄大鼠肝细胞再生与凋亡的影响。方法:40只大鼠随机分为正常组、模型组(生理盐水,10ml/kg)和复方葛根片低、中、高剂量组[200、400、800 mg(生药)/kg],每组8只。除正常组外,其他各组大鼠每天上午9:00 ip D-半乳糖溶液[250 mg/(kg·d)]复制衰老模型,每天下午15:00 ig相应药物,连续给药8周。按相关试剂盒操作分别测定血清、肝脏中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量以及肝脏中脂褐素(LPF)含量,并观察各组大鼠肝脏组织病理变化。结果:与正常组比较,模型组大鼠血清和肝组织中SOD活性显著降低,MDA、LPF含量显著升高(P<0.01);肝脏淋巴细胞显著增加,散在凋亡细胞增多,淋巴细胞在汇管区灶性浸润、肝血窦散在浸润。与模型组比较,复方葛根片低、高剂量组大鼠血清中SOD活性显著升高(P<0.05或P<0.01);中、高剂量组大鼠血清中MDA含量显著降低(P<0.01),肝组织中SOD活性显著升高(P<0.05或P<0.01);低、中、高剂量组大鼠肝组织中MDA、LPF含量显著降低(P<0.05或P<0.01);肝脏淋巴细胞减少,凋亡细胞少见,双核细胞显著增多。结论:复方葛根片能增加老龄大鼠肝细胞再生,减少其凋亡。OBJECTIVE ： To investigate the effects of Compound puerarin tablet on the regeneration and apoptosis of liver cells in aged rats. METHODS： 40 rats were randomly divided into normal group, model group （normal saline, 10 ml/kg）, Compound puerarin tablet low-dose, medium-dose and high-dose groups [200, 400, 800 mg （crude drug）/kg], 8 for each group. Except for normal group, aging model was induced in those groups by irrigation gastric administration of D-galactose solution [250 mg/（kg, d）] at 9 o＇clock every morning; they were given relevant medicine at 15 o＇clock every afternoon, ig, for 8 weeks. The contents of SOD and MDA in serum and liver, and the content of hepatic lipofuscin （LPF） in liver were determined by related kit operation procedure; the pathological change of liver tissue was observed in rats. RESULTS： Compared with normal group, the activity of SOD in serum and liver decreased significantly in model group, while the contents of MDA and LPF increased significantly （P〈 0.01） ; the number of leukomonocyte in liver of rats increased significantly, and scattered apoptotic cells increased; lymphocyte periportal focal and scattered infiltration of hepatic sinusoids were found in model group. Compared with model group, the activity of SOD in serum of rats increased significantly in Compound puerarin tablet low-dose and high-dose groups （P〈0.05 or P〈0.01 ）, while the content of MDA in serum of rats decreased significantly in medium-dose and high-dose groups （P〈0.01） ; the content of SOD in liver tissue increased significantly in medium-dose and high-dose groups （P〈0.05 or P〈0.01） , while the contents of MDA and LPF in liver tissue decreased significantly in low-dose, medium-dose and high-dose groups （P〈0.05 or P〈0.01） ; in low-dose, medium-dose and high-dose groups, the number of leukomonocyte decreased and apoptotic cells were rare, while the number of binucleate cells increased. CONCLUSIONS： Compound puerarin tablet can enhance liver cell regen

关 键 词：复方葛根片 抗衰老 肝脏 细胞再生 细胞凋亡 大鼠 

分 类 号：R285.5[医药卫生—中药学]