新疆北部主要盐生植物根部内生细菌群落结构的高通量分析  

Estimation of endophytic bacterial diversity in root of halophytes in Northern Xinjiang by high throughput sequencing

作　　者：赵帅[1] 周娜[1] 赵振勇[1] 张科[1] 吴国华 田长彦[1] Shuai Zhao Na Zhou Zhenyong Zhao Ke Zhang Guohua Wu Changyan Tian(State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, Xinjiang Uygur Autonomous Region, China Shizuishan Seed Management Station, Shizuishan 753000, Ningxia Hui Autonomous Region, China)

机构地区：[1]中国科学院新疆生态与地理研究所荒漠与绿洲生态国家重点实验室,新疆乌鲁木齐830011 [2]石嘴山市种子管理站,宁夏石嘴山753000

出　　处：《微生物学报》2016年第10期1583-1594,共12页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31300432);中国科学院西部青年学者项目(2015-XBQN-B-16);中国科学院"率先行动"计划特色研究所主要服务项目(TSS-2015-014-FW-3-1);中国科学院科技服务网络计划项目(KFJ-SW-STS-141)~~

摘　　要：【目的】揭示同一盐渍环境中不同种盐生植物根部内生细菌群落多样性特征和分布规律,结合根际土壤理化因子探讨其对内生细菌群落结构的影响。【方法】通过罗氏454高通量测序获得内生细菌16S r RNA片段,然后进行生物信息分析。【结果】研究的16种盐生植物其内生细菌群落主要由Proteobacteria、Tenericutes、Actinobacteria和Firmicutes 4个门的细菌组成。从植物"种"的水平来看,不同种盐生植物内生细菌群落存在差异;从植物"属"的水平来看,同一属的盐生植物内生细菌相似;从植物"科"的水平来看,藜科盐生植物内生细菌以Actinobacteria和Proteobacteria门为主;蒺藜科盐生植物内生细菌以Proteobacteria门为主;柽柳科盐生植物内生细菌以Tenericutes门为主;白花丹科盐生植物内生细菌以Proteobacteria、Fimicutes和Actinobacteria门为主。根际土壤中Cl~–含量对盐生植物内生细菌群落变化具有显著影响;而Cl~–、Mg^(2+)和总氮组成的集合与内生细菌群落结构相关性最高。【结论】盐生植物内生细菌多样性丰富。在同一盐渍生境中,盐生植物内生细菌群落分布呈现宿主的种属特异性,根际土壤中Cl~–是影响其内生细菌群落变化的主要驱动因素之一。[Objective] We studied the diversity of endophytic bacterial communities in different species of halophytes growing in the same saline habitat, and analyzed the effect of rhizosphere soil physicochemical properties on endophytic bacterial communities. [Methods] PCR-based Roche FLX 454 pyrosequencing was applied to reveal the diversity of endophytic bacteria. [Results] Endophytic bacterial communities of the 16 species of halophytes mainly included 4 phyla, which were Proteobacteria, Tenericutes, Actinobacteria and Firmicutes. In terms of plant species classification, colonial differences existed among plant species at perspectives of composition of bacterial taxa; in the case of plant genus level, endophytic bacteria of different halophyte plant species but belonging to same plant genus exhibited similarity; as to plant family level, Actinobacteria and Proteobacteria comprised the main abundant phyla of the halophytes belonging to Chenopodiaceae; Proteobacteria comprised the main abundant phyla of the halophytes belonging to Zygophyllaceae; Tenericutes comprised the main abundant phyla of the halophytes belonging to Tamaricaceae; Proteobacteria, Fimicutes and Actinobacteria comprised the main abundant phyla of the halophytes belonging to Plumbaginaceae. The CI- in rhizosphere soil has significant effect on endophytic bacterial community structure. Moreover, there is a strong correlation between bacterial community and the combination of CF, Mg2＋ and total nitrogen. [Conclusion] Halophytes harbors diverse endophytic bacteria. In the same saline habitat, the distribution of endophytic bacteria showed host plant species-specific, and the CF in rhizosphere soil was one of the factors determined the endophytic bacterial community.

关 键 词：盐生植物 内生细菌 多样性 焦磷酸测序 

分 类 号：Q948.122.3[生物学—植物学]