阿托伐他汀对动脉粥样硬化家兔血脂和脂质代谢因子的影响  

Effects of atorvastatin on atherosclerosis blood lipid and lipid metabolism factor synthesis in atherosclerotic rabbits

作　　者：张全武[1] 刘芮菡[1] 司金超[2] 

机构地区：[1]郑州大学附属郑州中心医院病理科,郑州450007 [2]郑州大学第二附属医院

出　　处：《医药论坛杂志》2016年第6期4-6,9,共4页Journal of Medical Forum

基　　金：河南省科技厅重点科技攻关项目(142102310461)

摘　　要：目的研究阿托伐他汀对动脉粥样硬化家兔主动脉斑块的影响及其可能的机制。方法 18只雄性日本大耳白兔随机分为3组:普通饲料组、高脂饲料组、高脂饲料+阿托伐他汀组,每组各6只。喂养16周后,采集各组家兔血清、主动脉及肝脏。检测各组家兔血清中血脂水平及主动脉病变的变化;提取各组胸主动脉及肝脏组织总RNA;实时定量PCR检测各组织内内脏脂肪素(Visfatin)、沉默信息调节因子1(Sirt1)、Bcl-2、C-myc及P53的mRNA水平变化。结果和对照组相比,高脂组和阿托伐他汀组家兔TC、LDL-C升高(t值分别为47.535、12.011,P值均为0),但阿托伐他汀组较高脂喂养家兔的血胆固醇(TC)和低密度脂蛋白(LDL-C)显著降低(t值分别为10.245、11.765;P值均<0.001);与高脂组相比,阿托伐他汀组家兔主动脉visfatin的mRNA水平下降(t=10.327,P<0.001),Bcl-2水平升高(t=7.042,P=0.001),肝脏组织中visfatin和p53的mRNA水平下降(t值分别为6.735,6.452;P=0.001),sirt1mRNA水平升高(t=8.328,P=0.001)。结论阿托伐他汀能够降低高血脂水平和抑制动脉粥样硬化,机制可能与降低Visfatin,p53表达及促进Sirt1,Bcl-2表达有关。Objective To investigate the effects of atorvastatin on aortic atherosclerotic and its possible mechanism. Methods Eighteen male Japanese white rabbits were randomly divided into three groups： normal diet group, high -fat diet group, high -fat diet with atorvastatin group, each group six rabbits. Sixteen weeks later, we collected each group rabbit＇ s serum, the aorta, and the liver for detecting each group rabbit＇ s serum lipid levels and aortic lesions and endarterium thickness changes. The expressions of visfatin, Sirtl, Bcl - 2, C - myc and 1）53 mRNA level in aorta and liver tissues by real time quantitative PCR. Results Compared with those in the normal diet group, levels of lipid in high - fat diet group and atorvastatin group were elevated（P 〈 0. 001 ）. Furthermore, compared with highfat diet group, the group of atorvastain rabbit＇ s aorta visfatin mRNA level declined and the Bcl - 2 elevated obviously （ P 〈 0. 001, P = 0. 001 ）, visfatin and p53 mRNA level declined and Sirtl elevated in liver tissue, respectively （P = 0. 001 ）. Conclusion Atorvastatin as a kind of hypolipidemics could retards atherosclerosis. Its possible mechanism was that atorvastatin reduced the expression of Visfatin, p53 in vascular tissues and promoted the expression of Sirtl, Bcl -2.

关 键 词：动脉粥样硬化 阿托伐他汀 内脏脂肪素 沉默信息调节因子1 BCL-2 

分 类 号：R332[医药卫生—人体生理学]