参芪归芍汤化学成分的UPLC-Q-TOF/MS分析  

Analysis on chemical components of Shenqi Guishao decoction by UPLC-Q-TOF/MS

作　　者：刘静[1] 陈鹏[1] 陈林伟 王琴[2] 吴红旗[2] 徐蓉[2] 

机构地区：[1]西安外事学院医学院,西安710077 [2]泰州市中医院,泰州225300

出　　处：《药物分析杂志》2016年第9期1578-1584,共7页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用超高效液相色谱-飞行时间质谱(UPLC-Q-TOF/MS)对参芪归芍汤中的化学成分进行分析和鉴定。方法:采用ACQUITYTM UPLC BEH C18(100 mm×2.1 mm,1.7μm)色谱柱,以0.1%甲酸-乙腈为流动相进行快速梯度洗脱,流速1.0 m L·min^(-1),柱温35℃,进样量10μL。电喷雾离子源负离子模式,毛细管电压分别为1.4 k V和1.3 k V,锥孔电压分别为40 V和23 V,离子源温度120℃,脱溶剂气温度350℃,锥孔气流量50 L·h^(-1),脱溶剂气流量600 L·h^(-1),碰撞能量10~40 e V,离子能量1 e V。结果:从参芪归芍汤中鉴定了42个化合物,结果显示,参芪归芍汤中的主要化学成分包括皂苷、黄酮和酚酸等类化合物。结论:该研究比较全面地阐明了参芪归芍汤的化学组成,对参芪归芍汤的质量控制和药效物质基础的研究具有十分重要意义。Objective：To analyze and identify the chemical constituents of Shenqi Guishao decoction by ultra performance liquid chromatography time-of-flight mass spectrometry（UPLC-Q-TOF/MS）. Methods：The separation was performed on an ACQUITYTM UPLC BEH C18 Column（100 mm×2.1 mm,1.7 μm）with gradient elution,which was carried out with 0.1% formic acid acetonitrile as mobile phase. The flow rate was 1.0 m L·min^（-1）,the temperature of column was 35 ℃,and the injection volume was 10 μL. Electrospray ionization source was in negative ion mode,capillary voltage was 1.4 k V and 1.3 k V,cone voltage was 40 V and 23 V,ion source temperature was 120 ℃,removal of solvent gas temperature was 350 ℃,cone gas flow was 50 L·h^（-1）,de-solvation gas flow was 600 L·h^（-1）,collision energy was 10 to 40 e V,and the ion energy was 1 e V. Results：Forty-two peaks were identified on line using the established method. The results implied that saponins,flavonoids and phenolic acids were main components of Shenqi Guishao decoction. Conclusion：The study has comprehensively illustrated the chemical composition of Shenqi Guishao decoction,which is significant to the quality control and the study of material basis of efficacy of Shenqi Guishao decoction.

关 键 词：参芪归芍汤 人参 黄芪 当归 炒白芍 皂苷类 黄酮类 酚酸类 结构鉴定 超高效液相色谱-飞行时间质谱 

分 类 号：R917[医药卫生—药物分析学]