一种毛尖茶叶多糖MTP06的提取分离及其活性测定  

Purification and Activity Determination of Polysaccharides MTP06 from Maojian Tea

作　　者：范海涛[1,2] 刘庄[3] 张照康 程湘懿 乔善义[2] FAN Haitao LIU Zhuang ZHANG Zhaokang CHENG Xiangyi QIAO Shanyi(College of Bioengineering, Bei jing Polytechnic, Beijing 100176, China Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences , Bei jing 100850, China Hospital of Beijing Union University, Beijing 100101, China Department of Physics and Institute of Biophysics, Central China Normal University, Wuhan 100191, China)

机构地区：[1]北京电子科技职业学院生物工程学院,北京100176 [2]军事医学科学院毒物药物研究所,北京100850 [3]北京联合大学校医院,北京100101 [4]华中师范大学物理科学与技术学院生物物理研究所,湖北武汉100191

出　　处：《茶叶科学》2016年第5期531-536,共6页Journal of Tea Science

基　　金：北京电子科技职业学院重点课题项目资助;国家"重大新药创制"科技重大专项(2013ZX09508-105)资助

摘　　要：本研究对一种毛尖茶叶多糖的结构与活性开展了研究。采用水提醇沉法提取毛尖茶叶粗多糖,经除蛋白后得到精制多糖(MP),以不同的柱层析方法对MP进行多次分离纯化,得到1个均一组分的毛尖茶叶多糖(Maojian Tea Polysaccharides No.06,MTP06)。采用1,1–二苯基–2–三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl,DPPH)自由基清除、小鼠免疫细胞RAW264.7增殖、吞噬能力和产生NO等试验方法对MTP06的活性进行研究。结果显示MTP06对0.1 mmol·L^(-1) DPPH溶液的自由基清除率为30.85%,对小鼠巨噬细胞RAW264.7的增殖、吞噬能力和产生NO的能力均有促进作用,与空白对照组间有显著差异(P<0.01),且质量浓度为500 mg·L^(-1)时,活性最大。To investigate the structure and activity of polysaccharides （MTP06）, the polysaccharides were extracted and purified from Maojian tea. The refined Maojian tea polysaccharides （MP） were obtained after removing proteins. Different column chromatography methods were used for several times to purify the MP and an uniform polysaccharide named MTP06 was obtained. The effects of MTP06 on DPPH radical-scavenging activity, cell viability, engulfment capability and NO production of RAW264.7 macrophages were investigated . The results showed that the free radical scavenging rate of MTP06 to 0.1 mmol·L^-1 DPPH solution was 30.85%. The highest viability, engulfment capability and NO production of RAW264.7 macrophages had been stimulated by MTP06 at 500 mg·L^-1. The present results confirmed MTP06 can eliminate DPPH radicals and stimulate cell viabilities.

关 键 词：毛尖茶叶 多糖 活性 免疫细胞 

分 类 号：TQ281[化学工程—有机化工]