新疆鼠尾草二萜醌类成分含量测定及抗血小板聚集作用  

Determination of Diterpenoid Quinone in Salvia deserta Roots and Its Inhibition for Platelet Aggregation

作　　者：王新玲[1] 王小青[1] 胡君萍[1] 王晓梅[1] 热娜.卡斯木[1] 崔智慧[1] 

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011

出　　处：《中国实验方剂学杂志》2016年第19期48-52,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：新疆维吾尔自治区高校科研计划科学研究重点项目(XJEDU2012I23)

摘　　要：目的：研究新疆鼠尾草中荷茗草醌和7-氧-乙酰荷茗草醌抗血小板聚集作用,并建立同时测定其中荷茗草醌,7-氧-乙酰荷茗草醌和6,7-去氢罗列酮3个主要有效成分含量的方法。方法：应用比浊法测定荷茗草醌和7-氧-乙酰荷茗草醌体外抗血小板聚集作用;采用HPLC同时测定荷茗草醌,7-氧-乙酰荷茗草醌和6,7-去氢罗列酮的含量。结果：荷茗草醌和7-氧-乙酰荷茗草醌高、中剂量组抑制凝血酶诱导的血小板聚集作用与生理盐水组比较均有显著性差异,且与质量浓度呈明显的剂量依赖性;荷茗草醌,7-氧-乙酰荷茗草醌和6,7-去氢罗列酮进样量分别在0.31～4.56μg（r=0.999 6）,0.01～0.24μg（r=0.999 4）,0.4～6.0μg（r=0.999 5）与峰面积呈良好的线性关系;平均加样回收率分别为100.3%（RSD 2.0%）,100.6%（RSD 3.1%）,102.5（RSD 1.6%）。结论：新疆鼠尾草中荷茗草醌和7-氧-乙酰荷茗草醌表现出显著抑制血小板聚集作用;建立的HPLC含量测定方法简便快速,结果准确可靠、重复性好,可用于新疆鼠尾草药材的质量控制与评价。Objective： To evaluate the anti-platelet aggregation effect of horminone and 7-Oacetylhorminone in roots of Salvia deserta,and to establish a method for simultaneous determination of three effective constituents（ horminone, 7-O-acetylhorminone and 6, 7-dehydroroyleanone） in S. deserta roots.Method： In vitro anti-platelet aggregation of horminone and 7-O-acetylhorminone was determined by turbidimetry.The contents of horminone,7-O-acetylhorminone and 6,7-dehydroroyleanone were simultaneously determined by HPLC. Result： As compared with the normal saline group,horminone and 7-O-acetylhorminone of the high and middle dose groups could effectively inhibit platelet aggregation induced by thrombin in a clear dose-dependent manner. The horminone,7-O-acetylhorminone and 6,7-dehydroroyleanone showed good linear relationship with the peak area within 0. 31-4. 56 μg（ r = 0. 999 6）,0. 01-0. 24 μg（ r = 0. 999 4） and 0. 4-6. 0 μg（ r =0. 999 5）; their average recoveries were 100. 3%（ RSD 2. 0%）,100. 6%（ RSD 3. 1%）,and 102. 5%（ RSD1. 6%） respectively. Conclusion： Horminone and 7-O-acetylhorminone in roots of S. deserta showed better antiplatelet aggregation effect. The method of HPLC is simple,accurate and repeatable foe content determination,and can be used for quality control and evaluation of S. deserta.

关 键 词：新疆鼠尾草 高效液相色谱法 荷茗草醌 6 7-去氢罗列酮 

分 类 号：R284.1[医药卫生—中药学]