“从肠论治”对急性肺损伤大鼠P物质含量影响  

Influence of “Treatment Based on Large Intestine” on Contents of Substance P in Rats with Acute Lung Injury

作　　者：童晓萍[1] 何德平[1] 林琳[1] 

机构地区：[1]广东省中医院,广东广州510006

出　　处：《辽宁中医药大学学报》2016年第9期33-35,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：广东省中医药局科研课题(20141096)

摘　　要：目的:通过了解宣白承气汤与其拆方对内毒素诱导的急性肺损伤(ALI)大鼠肺和大肠组织中P物质的影响,从神经肽方面去研究ALI"从肠论治"的有效机理。方法:35只雄性Wistar大鼠随机分为对照组、治肺组、治肠组、肺肠同治组、模型组。采取气管内注入脂多糖的方法制备ALI大鼠模型。各治疗组在造模前相应药物灌胃6 d,而对照组和模型组在相应时间点予等容积生理盐水灌胃,第6天灌胃后0.5 h造模,造模4 h后测肺组织湿/干重比(W/D),用ELISA法检测肺、大肠组织中P物质的含量,并观察肺组织病理变化。结果:与对照组相比,模型组大鼠肺组织的W/D升高(P<0.05),模型组大鼠肺组织P物质含量明显升高,肠组织里的P物质显著降低(P<0.01);对比模型组,治肠组、肺肠同治组W/D下降(P<0.05),治肺组、治肠组、肺肠同治组肺组织中的P物质含量都显著降低(P<0.01),肠组织中的P物质都显著升高(P<0.01或P<0.05);对比治肺组,肺肠同治组肺组织中P物质显著降低(P<0.01),而肠组织中P物质却升高(P<0.05);光镜下模型组呈现ALI的病理改变,而各治疗组较模型组均有改善,肺肠同治组改善较明显。结论:通利大肠或在治肺的同时增进通利大肠,会改变ALI大鼠肺及大肠组织中P物质含量,这有可能是"从肠论治"ALI有效的机理之一。Objective：To abserve the influence of Xuanbai Chengqi （XBCQ）Decoction and its split prescription on contents of substance P （ SP ） in lung and large intestine tissue of acute lung injury （ALI ） rats induced by LPS, and explore the effective mechanism of ＂treatment based on large intestine＂ in the view of neuropeptide. Methods ： Thirty-five male Wistar rats were randomly divided in to control group, lung group, intestine group, lung-intestine group and model group. Rats model of ALI were established by intratracheal instillation of LPS. The rats in each treatment group were pretreated by corresponding drugs for 6 days before LPS-induced ALL The control group and model group were intragastrically given normal saline solution at the same time. The ALI model were set up half an hour after treatement at the sixth day. All the rats were detected lung wet/dry weight ratio and observed pathologic variety of the lung tissue after 4 hours of induced ALI model. The contents of SP in lung and colonic tissue was detected by enzyme-linked immunosorbent assay. Results ： Compared with the control group, the lung wet/dry weight ratio increased in model group （ P〈0.05 ） and contents of SP in lung increased significantly （ P〈0.01 ）, while SP in intestine decreased broadly （~P〈0.05）; compared with model group, the lung wet/dry weight ratio decreased in intestine group and lung-intestine group （ P〈O.05 ）, contents of SP in lung decreased significantly （ P〈0.01 ） in intestine group, lung group and lung-intestine group, while contents of SP in intestine increased （ P〈0.01 or P〈0.05 ）. Compared to lung group, contents of SP in lung decreased significantly （ P〈0.01 ） in lung- intestine group, while contents of SP in intestine increased （ P〈0.05 ）. The ALI pathological change can be found in model group by light microscope, while the pathological change were improved in each treatment group, especially in lung-intestine group. Conclusion ： Contents of SP in lung and

关 键 词：急性肺损伤 从肠论治 P物质 

分 类 号：R563[医药卫生—呼吸系统]