紫地榆防龋活性筛选及其防龋机制初步研究  

Preliminary study of Geranium Strictipes R. Knuth anticarie activity and anticaries mechanism

作　　者：汪春平 骆婷婷[1] 吴利先[2] 王涛[2] 蓝海[1] 

机构地区：[1]大理学院药学与化学学院,671000 [2]大理学院基础医学院

出　　处：《实用口腔医学杂志》2016年第5期620-623,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81260512);云南省大理学院应用开发研究基(编号:Kyyy201102)

摘　　要：目的:研究紫地榆各提取物防龋活性和活性成分对口腔致龋菌的增殖抑制以及产酸、产多糖功能的影响。方法:以洗必泰和五倍子作为阳性对照,采用试管二倍稀释法研究紫地榆提取物对2种主要致龋菌生长和代谢的影响,先测定紫地榆5种提取物对变形链球菌(S.mutans)、黏性放线菌(A.viscosus)的最低抑菌浓度(MIC)及最低杀菌浓度(MBC),从中筛选出防龋活性部位;再测定低于MIC的4个浓度的紫地榆防龋活性部位对2种致龋菌产酸、产多糖的影响。结果:紫地榆正丁醇萃取部位对2种致龋菌抑制效果最好,对细菌产酸、产多糖均有一定的抑制作用,但作用弱于洗必泰和五倍子。结论:紫地榆正丁醇萃取部位可抑制致龋菌生长代谢,可能是一种天然防龋药物。Objective： To screen the different extracts from Geranium strictipes with anticaries activity,and to study the effect of the active extract on the production of acid and polysaccharides of oral cariogenic bacteria. Methods： Chlorhexidine and Galla Chinensis were used as the positive controls. Tube double dilution method was used to study the extracts of Geranium strictipes on the growth of Streptococcus mutans （ S. mutans ） and Actinomyce viscosus （ A. viscosus ）. First, the activity of 5 extracts fom Geranium strictipes against S. mutans, A. viscosus was detected. Their minimum inhibitory concentration （ MIC ） and minimum bactericidal concentration （ MBC ） were obtained. The effects of 4 lower concentrations（ 〈 MIC） of the active extract on the production of oral bacterial acid polysaccha- rides were examined. Results： The effect of n-butanol extraction part of Geranium strietipes was the strongest among all the extracts a- gainst the two kinds of bacteria in the inhibition of bacteria growth, bacterial acid production and polysaccharides production, but weaker than that of Chlorhexidine and Galla Chinensis. Conclusion： N-butanol extraction part of Geranium strictipes may be a natural and effective anti-caries agent.

关 键 词：紫地榆 致龋菌 生长 产酸 产多糖 

分 类 号：R285[医药卫生—中药学]