欧美杨与藏川杨杂交子代苗期性状QTLs定位分析  

Mapping of QTLs in Hybrid Progeny of Populus euramericana and Populus szechuanica var. tibetica

作　　者：李娟[1] 郭斌[1,2] 安新民[1] 

机构地区：[1]北京林业大学林木育种国家工程实验室,北京100083 [2]山西省林业科学研究院,山西太原030012

出　　处：《西南林业大学学报（自然科学）》2016年第5期10-15,共6页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家"十二五"科技支撑项目(2012BAD01B0302)资助

摘　　要：以欧美杨I-108和藏川杨为亲本杂交得到427个子代为试材,对其进行苗期性状遗传图谱分析,并构建了1张总长度969.1 c M,标记间平均距离25.14 c M的杂交杨树遗传图谱。结果表明:该图谱包含6个连锁群,连锁群上标记数量37个,用Genemap软件绘制标记连锁图谱;检测到株高(h_s)、单叶光合速率(P_n)、胞间CO_2浓度(C_i)、蒸腾速率(T_r)4个表型指标符合正态分布。利用MAPMAKER软件的区间作图法进行QTL定位和效应估计,共检测到8个与苗期生长和光合性状相关的QTL,包括3个与h_s相关的QTL,贡献率分别为16.4%、20.1%和17.8%;2个与P_n相关的QTL,贡献率分别为10.2%和13.7%;2个与C_i相关的QTL,贡献率分别为6.7%和8.3%;1个与T_r相关的QTL,贡献率9.7%。A population including 427 germplasm resources, crossed by female parent Populus euramericana 1- 108 and male parent Populus szechuanica var. tibetica were used to construct the genetic linkage map in seedling stage. The map contains 37 SSR makers and 6 linkage groups spanning 969. 1 cM with an average of 25.14 cM be- tween markers. Genemap software was used to draw the marker linkage map. The four phenotype indicators of height （ ha） , single leaf photosynthetic rate （ Pn ） , intercellular CO2 concentration （ Ci ）, transpiration rate （ Tr ） are in line with normal distribution. The interval mapping method of software package MAPMAKER were used to map an- danalyze QTLs. 8 QTLs were detected for seedling growth and photosynthetic trait. 3 QTLs were detected for seed- ling height which explained 16.4%, 20. 1% and 17.8% of the phenotypic variation. 2 QTLs were detected for pho- tosynthetic rate which explained 10. 2% and 13.7% of the phenotypic variation. 2 QTLs were detected for Intercel- lular CO： concentration which explained 6.7% and 8.3% of the phenotypic variation. 1 QTL was detected for tran- spiration rate which explained 9.7% of the phenotypic variation.

关 键 词：藏川杨 欧美杨 杂交 遗传图谱 QTL定位 苗期性状 

分 类 号：S722.3[农业科学—林木遗传育种]