基于高效液相色谱法对不同提取条件下竹叶黄酮中牡荆苷含量的分析  

Analysis for Vitexin Content of Bamboo Leaves Flavonoids in Different Extraction Condition with High Performance Liquid Chromatography(HPLC)

作　　者：华梅[1] 谭芮[1] 王毅[1] 缪福俊[1] 陈剑[1] 马惠芬[1] 杨宇明[1] 王娟[1] 

机构地区：[1]云南省林业科学院,云南省森林植物培育与开发利用重点实验室,国家林业局云南珍稀濒特森林植物保护和繁育重点实验室,云南昆明650201

出　　处：《西南林业大学学报（自然科学）》2016年第5期164-168,共5页Journal of Southwest Forestry University：Natural Sciences

基　　金：云南省应用基础研究重点项目(2013FA054);云南省科技计划(对外科技合作计划)(2014IA013);云南省科技计划(对外科技合作计划-国际科技合作)(2015IA005);云南省应用基础研究计划青年项目(2014FD069);国家林业公益性行业科研专项项目(201204110);云南省重点实验室开放基金;亚太森林网络(APFNet)-联合国大学(UNU)跨国森林植被恢复项目(600UU-1123)

摘　　要：用高效液相色谱对竹叶黄酮中牡荆素进行分析,并对其含量进行了测定,同时研究了提取溶剂比、料液比和提取时间对牡荆素含量的影响。结果表明:最佳的提取条件为,提取溶剂比为70%乙醇水,料液比为1∶20,提取时间为2.0 h。采用牡荆苷作为外标物,建立牡荆苷标准曲线方程:m=4.725×10-7A+0.042 5(R2=0.999)。在最佳的提取条件下,采用大孔树脂对提取物进行纯化,对竹叶黄酮中牡荆素的含量进行测定,灰金竹中牡荆素的含量为(1.01%±0.07%)。We studied the Vitexin in flavonoids of bamboo leaves and measured the content with HPLC exter- nal standard method, simultaneously investigated the influences of extraction solvent ratio, solid to liquid ratio, and extraction time on the content of Vitexin in flavonoids of bamboo leaves. The results showed that the optimum ex- traction conditions were： extraction solvent ratio was 70% ethanol-water, solid-liquid ratio was 1：20, extraction time was 2. 0 h. We established the standard curve equation m = 4. 725 10^-7A ＋ 0. 042 5 （ R2 = 0. 999）, using Vi- texin as external standard substance and purifying extract with Macroporous resin. Under the optimum extraction conditions, the Vitexin content in Phyllostachys nigra var. henonis Stapf ex Rendle was （ 1.01% ± 0. 07% ）.

关 键 词：竹叶黄酮 牡荆素 提取条件 外标法 高效液相色谱 

分 类 号：S795[农业科学—林木遗传育种] Q946.8[农业科学—林学]