手术引发应激导致心律失常大鼠模型的创建  

Establishment of Arrhythmia Models in Rats Based on the Stress Response Caused by Surgical Trauma

作　　者：庄儒麟 李梅[2] 李华伟 王莹莹 郝清涛 胡京剑 杨超 戴梅清 马行[2] 

机构地区：[1]徐州医科大学临床医学系,221004 [2]徐州医科大学药学院药理学教研室,221004 [3]徐州医科大学公共卫生学院,221004

出　　处：《医学研究杂志》2016年第9期88-90,87,共4页Journal of Medical Research

基　　金：江苏省高等学校大学生创新创业训练计划省级重点项目(201410313015Z)

摘　　要：目的初步创建手术创伤引发应激反应导致心律失常的大鼠模型,探讨在不同方法下建立的大鼠心律失常模型是否存在差别,为今后临床药物筛选和药效评价提供新的研究依据。方法选择SD大鼠30只,分别采用探查腹腔暴露肠道法(9只)、夹闭肠系膜上动脉法(14只),以及单侧颈总动脉结扎法(7只)进行手术,采用Powerlab生物功能实验系统监测动物心电图的方法,观察、比较并记录大鼠整个手术过程的心电图。结果采用探查腹腔暴露肠道法进行手术,大鼠心率明显减慢,手术组和对照组间差异无统计学意义(P>0.05)。采用夹闭肠系膜上动脉法进行手术,大鼠肠系膜上动脉夹闭15min后,心电图显示心肌缺血,夹闭20min后,心电图可见QRS波群增宽,手术组和对照组间差异有统计学意义(P<0.05)。采用单侧颈总动脉结扎法进行手术,大鼠单侧颈总动脉结扎15min后,心电图呈现QRS波群增宽,心率明显减慢,手术组和对照组间差异有统计学意义(P<0.05)。结论相比于探查腹腔暴露肠道法和夹闭肠系膜上动脉法,单侧颈总动脉结扎法在上述3种方法中较为稳定,在结扎15min后,大鼠出现心律失常,即为造模成功。Objective To establish arrhythmia models in rats based on the stress response caused by surgical trauma preliminarily, to discuss whether there is a difference between the arrhythmia models in rat based on different methods, in order to provide new experimental evidence for clinical drug screening and pharmacodynamics evaluating in the future. Methods Firstly, we selected the SD rats and then did some operation on them respectively using three methods： exploring the exposure abdominal,occluding superior mesenteric artery and ligating common carotid artery unilaterally. Finally, we observed and compared the ECG recorded by the Power lab. Results Exploring the exposure abdominal in rats can result in decreasing of heart rate significantly, but there was no difference between operation group and control group. Occluding superior mesenteric artery in rats can result in myocardial ischemia after 15min and wide QRS after 20min in ECG. And there was a significant difference between the operation group and the control group. Ligating common carotid artery unilaterally can result in wide QRS and heart rate decreasing significantly after 15rain. And there was a significant difference between the operation group and the control group, too. Conclusion Compared with exploring the exposure abdominal and occluding superior mesenteric artery in rats, ligating common carotid artery unilaterally is the most stable. Arrhythmia was observed in all of rats after ligating 15min, which testified the success of the model.

关 键 词：动物模型 大鼠 手术应激 心律失常 心肌缺血 

分 类 号：R965.1[医药卫生—药理学] R972.2[医药卫生—药学]