昆虫肠道共生菌中产γ-氨基丁酸菌株的筛选及鉴定  

Screening and identify of symbiotic bacteria producing γ-aminobutyric acid from insect gut

作　　者：唐帅[1] 王艳新[1] 刘开新[1] 夏婷婷[1] 金海如[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《浙江师范大学学报（自然科学版）》2016年第3期320-324,共5页Journal of Zhejiang Normal University:Natural Sciences

基　　金：国家自然科学基金资助项目(41371291);浙江省自然科学基金资助项目(LY12C01002)

摘　　要：从白蚁、蜜蜂、蝗虫、蜻蜓等10种昆虫肠道中分离得到昆虫肠道共生菌51株,采用薄层色谱法初筛、高效液相色谱法复筛,获得了多株产γ-氨基丁酸(GABA)的菌株.对其中一株GABA产量较高的菌株FE-7进行了显微形态观察、生理生化与16S r DNA扩增序列系统发育分析,鉴定FE-7可能为芽孢杆菌属的一个新种,其发酵液中GABA含量初测为2.1 g/L以上.表明从昆虫肠道特境中筛选产γ-氨基丁酸菌株具有开发前景.51 intestinal symbiotic strains were isolated from 10 species of insect gut samples （termites, bees, locusts, dragonflies etc. ）. Several γ-aminobutyric acid producing strains were obtained by screening com- prised approach of TLC, HPLC. The high-yield γ-aminobutyric acid producing strain FE-7 was identified as a possible new species of the genus bacillus based on microscopic morphology observation, physiological and biochemical characteristics and 16S rDNA sequence amplification phylogenetic analysis. The content of GABA was over 2.1 g/L in the raw fermented liquid. Thereby, screening of symbiotic bacteria producing γ-aminobu- tyric acid from special intestinal condition of insect would be useful and prospective development.

关 键 词：共生菌 昆虫肠道 Γ-氨基丁酸 筛选 鉴定 

分 类 号：Q939.99[生物学—微生物学]