蚂蟥冬眠临界温度的研究  

Studies on hibernation pivotal temperature of Whitmania pigra

作　　者：闫士猛 史红专[1] 郭巧生[1] 王嘉[1] 吴斌[1] 施国伟[1] 李蒙蒙[1] 戴道新[1] 吴曼郡 YAN Shi-meng SHI Hong-zhuan GUO Qiao-sheng WANG Jia WU Bin SHI Guo-wei LI Meng-meng DAI Dao-xin WU Man-jun(Institute of Chinese Medicinal Materials, Nanfing Agricultural University, Nanjing 210095, China)

机构地区：[1]南京农业大学中药材研究所,江苏南京210095

出　　处：《中国中药杂志》2016年第19期3615-3619,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81473306)

摘　　要：采用生物学观测法、静水法、3,5-二硝基水杨酸比色法、对棕榈酸硝基苯酯(ρ-NPP)比色法、福林-酚法观测和测定不同温度下蚂蟥生物学特征、耗氧量、耗氧率、消化道淀粉酶、脂肪酶和蛋白酶的活性。结果表明,随着温度的降低,蚂蟥活动减弱、进食减少,4℃后不食不动,头部和尾部弯曲呈"弯月"状;耗氧量、耗氧率以及消化道酶活性随温度下降逐渐下降,10℃后下降趋势减缓,4℃后趋于稳定,不随温度降低而变化;在4℃状态40 d过程中,淀粉酶变化不显著,脂肪酶、蛋白酶活性在20d时有所降低,其中脂肪酶下降后又轻微上升后保持较低水平。结论,4℃为蚂蟥冬眠的临界温度,"弯月"状可作为其冬眠状态的标志。The biological characteristics,oxygen consumption,oxygen consumption rate,and activities of amylase,lipase and protease of Whitmania pigra at different temperature were studied by using direct observational method,the still water method and 3,5-dinitrosalicylic acid colorimetry,right-nitrophenyl palmitate ester（ ρ-NPP） colorimetry and folin-phenol method. The results revealed that with decreasing water temperature,the daily activity and the daily feeding ration were decreased. As the temperature was lowered to 4℃,the head and tail of Wh. pigra curved,showing a crescent-shape without feeding and daily activity. Oxygen consumption,oxygen consumption rate and digestive enzyme activities reduced along with temperature drops. The downward trend slowed below 10 ℃,began to stabilize below 4 ℃ and doesn＇t change with the decrease of temperature since then. During the 40 days treatment at 4 ℃,the changes of amylase were not significant,the lipase and protease activity decreased at the 20 th day,and the lipase showed an slightly increase after the decrease and finally remained at a low level. In conclusions,the pivotal temperature of hibernation of Wh. pigra is 4 ℃and the crescent shape can be considered as a symbol of hibernation.

关 键 词：蚂蟥 冬眠临界温度 生物学特性 耗氧量 耗氧率 消化酶 

分 类 号：S917.4[农业科学—水产科学]