饲料VD3水平对黄颡鱼转录组差异基因表达的影响  

Effects of dietary vitamin D_3 level on transcriptomic response in yellow catfish(Pelteobagrus fulvidraco)

作　　者：冯美惠 陈沛[1] 段鸣鸣[1] 王春芳[1] FENG Meihui CHEN Pei DUAN Mingming WANG Chunfang(College of Fisheries, Huazhong Agricultural University, National Technology System for Conventional Freshwater Fish Industries, Hubei Provincial Engineering Laboratory for Pond Aquaculture, Freshwater Aquaculture Collaborative innovation Center of Hubei Province, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, Chin)

机构地区：[1]华中农业大学水产学院,大宗淡水鱼产业技术体系华中区养殖岗位,池塘健康养殖湖北省工程实验室,淡水水产健康养殖湖北省协同创新中心,农业部淡水生物繁育重点实验室,湖北武汉430070

出　　处：《水产学报》2016年第9期1289-1298,共10页Journal of Fisheries of China

基　　金：国家自然科学基金(31172421)~~

摘　　要：为研究饲料中添加维生素D_3对黄颡鱼基因表达的影响,实验基于RNA-Seq技术对用添加不同水平维生素D_3[0(VD0),1243(VD2),22700(VD20)IU/kg]的饲料喂养的黄颡鱼肾脏和小肠的转录组数据进行分析。通过对305 568982条原始reads的筛选和排除,得到了83 265条unigenes,平均长845.38 nt,N50为1620 nt。利用Blast等相关软件对数据进行深度分析,得到功能注释基因共29 224个。根据GO富集分析发现,差异表达基因主要集中在细胞过程、代谢通路、生物调控等通路中,KEGGpathway富集分析结果显示,代谢通路涉及基因最多,有1532个。相对于对照组,添加1243和22700 IU/kg的VD_3(VD0 vs VD2/VD0vs VD20)可得到共同上下调基因共380个,其中上调基因266个,下调基因114个;3种水平下共同上下调的基因共2 1个,其中上调基因4个,下调基因1 7个。研究表明,在饲料中添加不同浓度维生素D_3会对黄颡鱼的生长代谢和生物调控等相关基因表达产生影响,且不同浓度实验组,相关基因的表达存在差异。本实验通过对差异表达基因的后续分析及预测,为黄颡鱼的生长代谢及疾病预防等方面的研究提供了丰富的数据来源。The study was carried out to investigate the effects on possible gene regulation in intestine and kidney of yellow catfish（Pelteobagrus fulvidraco） under the treatments of different dietary vitamin D_3 levels（0（VD0）,1243（VD2）,22 700（VD20） IU/Kg） by RNA-seq technique.83 265 unigenes were selected from the 305 568 982 raw reads.The average length was 845.38 nt and N50 was 1620 nt.29 224 genes were annotated by using Blast software.According to the results of gene ontology functional enrichment analysis,significant differently expressed genes Were mainly distributed on the pathways like cellular process,metabolic pathways and biological regulation.The results of KEGG pathway enrichment analysis showed that 1532 genes were involved in metabolic pathway,in which the affected genes were the most.Dietary vitamin D treatment significantly affected the genes expression.When compared with the control group（VDO vs VD2/VD0 vs VD20）,380 genes were up-and downregulated in common,in which 266 genes were up-regulated and 114 genes were down—regulated.Among three levels（VDO vs VD2 vs VD20）,21 genes were up-and down-regulated in common,including 4 genes up regulated and 17 down-regulated.The results indicated that different dietary vitamin D_3 levels significantly affected the gene expressions in intestine and kidney of yellow catfish.The information of the differentially expressed genes provided evidences about the growth,metabolism and immunological response of yellow catfish.

关 键 词：黄颡鱼 维生素D_3 转录组分析 基因表达 

分 类 号：S963.7[农业科学—水产养殖]