胡萝卜肉桂醇脱氢酶基因的克隆及其对非生物胁迫的响应  

Cloning and Expression Profile Analysis of the Gene Encoding Cinnamyl Alcohol Dehydrogenase under Abiotic Stress in Carrot

作　　者：刘堰珺 马静[1] 王广龙[1] 黄蔚[1] 王枫[1] 熊爱生[1] LIU Yanjun MA Jing WANG Guanglong HUANG Wei WANG Feng XIONG Aisheng(State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, Nan- jing 210095, China)

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,园艺学院,农业部华东地区园艺作物生物学与种质创制重点实验室,南京210095

出　　处：《西北植物学报》2016年第7期1294-1301,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：江苏省自然科学基金杰出青年基金(BK20130027);江苏高校优势学科建设项目(PAPD)

摘　　要：该实验采用RT-PCR技术,从胡萝卜‘黑田五寸’中克隆获得了编码肉桂醇脱氢酶(Cinnamyl alcohol dehydrogenase,CAD)的基因DcCAD。DcCAD序列长1 074bp,编码357个氨基酸。亲水/疏水性分析表明,DcCAD属于亲水性蛋白。系统进化分析显示,DcCAD与番茄CAD(XP_010314515.1)亲缘关系最近。荧光定量PCR结果显示,DcCAD基因在胡萝卜根、叶片和叶柄中的表达量差异显著,其相对表达量为叶片>根>叶柄。DcCAD基因对高温(38℃)、低温(4℃)、干旱(20%PEG)和盐(0.2mol·L-1 NaCl)胁迫均有响应,尤其对高温胁迫和低温胁迫响应明显,而且高温处理后1h和低温处理后2h表达量最高。研究推测,DcCAD基因对胡萝卜抗逆性具有一定的作用。Here ,the gene encoding DcCAD were cloned from carrot cultivar ‘Kurodagosun’ with RT-PCR method .The length of DcCA D was 1 074 bp ,and encoding 357 amino acids .The DcCAD may belong to hydrophilic protein . Phylogenetic analysis showed that DcCAD closed to CAD from tomato （XP_010314515 .1） .Quantitative real-time PCR analysis showed that the expression profiles of the DcCA D gene are significantly different in different tissues （root ,leaf blade ,and petiole） of carrot .Relative expression level of the DcCA D gene is leaf blade〉 root〉 petiole .The DcCA D gene is responsive to various abiotic stresses ,such as heat （38 ℃） ,cold （4 ℃） ,drought （20% PEG） and salt （0 .2 mol？L -1 NaCl） .The ex-pression level was significantly increased after high temperature or low temperature treatments ,respective-ly .The expression levels peaked at 1 h heat treatment and 2 h cold treatment ,respectively .These results suggested that DcCA D gene participate in the response process of carrot under the abiotic stress .

关 键 词：肉桂醇脱氢酶 同源分析 非生物胁迫 表达分析 胡萝卜 

分 类 号：Q785[生物学—分子生物学] Q786