大豆SbPRP3基因表达及转基因烟草非生物胁迫鉴定  

Expression of SbPRP3 in Soybean and Resistance Ability in Transgenic Tobacco to Abiotic Stress

作　　者：翟莹[1] 张军[2] 杨晓杰[1] 赵艳[1] 陈阳[1] ZHAI Ying ZHANG Jun YANG Xiaojie ZHAO Yan CHEN Yang(College of Life Science and Agro-Forestry, Qiqihar Univesity, Qiqihar, Heilongjiang 161006, China Heilongiiang Institute of Veterinary Science, Qiqihar, Heilongjiang 161005, China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]黑龙江省兽医科学研究所,黑龙江齐齐哈尔161005

出　　处：《西北植物学报》2016年第7期1331-1336,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：黑龙江省教育厅科学技术研究项目(12541889)

摘　　要：为研究大豆(Glycine max L.)细胞壁脯氨酸富集蛋白基因(SbPRP3)在逆境胁迫中的作用,利用实时荧光定量PCR,对SbPRP3在高盐、干旱和低温处理下的表达情况进行检测。结果显示,SbPRP3在高盐处理下表达量升高,在干旱和低温处理下表达量先升高后降低。将SbPRP3构建到植物表达载体pRI101-AN上并转化烟草,获得阳性转基因烟草3株。对转基因烟草植株进行高盐、干旱和低温胁迫处理。结果表明,与对照相比,高盐和低温处理下转基因烟草脯氨酸积累量增加,而丙二醛产生量降低。但干旱处理后转基因烟草脯氨酸和丙二醛的含量与对照相比没有显著差异。由此推测SbPRP3可以提高转基因烟草的耐盐性和耐寒性。Plant proline-rich proteins （PRPs） are putative cell wall proteins ,which are usually associated with different abiotic stresses .The expression of SbPRP3 under abiotic stress treatments in soybean was detected by real-time fluorescence quantitative PCR .The expression of SbPRP3 increased under salt treat-ment ,w hile it increased firstly and thereafter declined under drought and cold treatments .Furthermore , the ORF of SbPRP3 was cloned into the plant expression vector of pRI101-AN and then introduced into to-bacco by Agrobacterium-mediated transformation .Three positive transgenic tobacco plants were obtained . The transgenic tobacco plants were treated with salt ,drought and cold stresses .The results showed that the transgenic plants maintained higher levels of proline and lower level of malondialdehyde compared to wild-type plants under salt and cold stresses .While the levels of proline and malondialdehyde in transgenic plants had no significant differences compared to wild-type plants under drought stress .These data indica-ted that the transgenic tobacco plants constitutively expressing SbPRP3 showed an increased tolerance to salt and cold compared to wild-type plants .

关 键 词：大豆 脯氨酸富集蛋白 SbPRP3 表达分析 抗逆性 

分 类 号：Q785[生物学—分子生物学] Q786