两种支架材料搭载骨髓间充质干细胞联合高压氧修复海水浸泡兔桡骨骨缺损  

BMSCs seeding in two scaffold incorporation with hyperbaric oxygen treat seawater immersed bony defect

作　　者：张淦[1] 程迅生[1] 陈聪聪[1] 陈肖松[1] 马武秀[1] 王崎[2] 符来想[2] Zhang Gan Cheng Xunsheng Chen Congcong et al(Dept of Orthopedics, 105 Hospital of PLA, Hefei 230031)

机构地区：[1]安徽医科大学解放军临床学院骨一科,合肥230031 [2]安徽医科大学解放军临床学院骨三科,合肥230031

出　　处：《安徽医科大学学报》2016年第10期1425-1429,共5页Acta Universitatis Medicinalis Anhui

基　　金：南京军区医学科技创新重点课题(编号:11Z011)

摘　　要：目的探讨n-HA/明胶、β-TCP/明胶搭载骨髓间充质干细胞(BMSCs)联合高压氧修复海水浸泡兔桡骨开放性骨缺损的效果。方法 45只新西兰大耳兔,在兔双侧桡骨中段制作15 mm的骨缺损区,双前肢人工海水浸泡3 h,制作成海水浸泡开放性骨缺损模型。将实验兔随机分成3组即对照组、n-HA/明胶组、β-TCP/明胶组,每组15只。伤口浸泡于人工海水3 h,彻底清创后,对照组在骨缺损区单纯植入BMSCs,n-HA/明胶组植入n-HA/明胶/BMSCs,β-TCP/明胶组植入β-TCP/明胶/BMSCs。手术后立即进行高压氧疗,每天1次,连续14 d。于手术后第4、8、12周摄片后分批处死后取材,行组织学和生物力学检测。比较各组缺损修复的效果。结果 (1)X线片检查:术后4周对照组、n-HA/明胶组、β-TCP/明胶组骨缺损区有薄云状骨痂阴影,阴影区域n-HA/明胶组>β-TCP/明胶组>对照组。术后8周,对照组少量骨痂形成;n-HA/明胶组、β-TCP/明胶组新生骨痂大量形成。术后12周,对照组骨缺损未完成修复;n-HA/明胶组、β-TCP/明胶组皮质连续性好,髓腔再通,基本完成修复;(2)骨痂灰度值测量:相同时间观察点,骨痂灰度值n-HA/明胶组>β-TCP/明胶组>对照组。术后第12周时,对照组、n-HA/明胶组、β-TCP/明胶组骨痂灰度值差异有统计学意义(P<0.05);(3)组织学观察:术后第4周,n-HA/明胶组、β-TCP/明胶组有新生骨小梁生成,骨小梁形成量n-HA/明胶组>β-TCP/明胶组>对照组,可见部分移植的支架材料残余;术后第8周,对照组未见板层骨形成,n-HA/明胶组、β-TCP/明胶组有大量板层骨形成,术后第12周,对照组少量板层骨,n-HA/明胶组、β-TCP/明胶组大量板层骨形成,两组移植的支架材料均消失;(4)生物力学检测:术后12周各组最大抗折力n-HA/明胶组>β-TCP/明胶组>对照组,n-HA/明胶组和β-TCP/明胶组抗折力接近于正常骨。结论 nHA/明胶/BMSCs联合高压氧和β-TCP/明胶/BMSCs联合高压氧均能修�Objective To investigate the efficiency of n-HA/gelatin and β-TCP/gelatin scaffold incorporation with HBO to enhance seawater immersed open bone defect. Methods A 15 mm radius defect was made in 45 rabbits, and then wound limbs were immersed in seawater for 3 h. The rabbit model of bone defect immersed by seawater was established. All rabbits were randomly divided into 3 groups. BMSCs were implanted in control group, n-HA/ gelatin/BMSCs were implanted into n-HA/gelatin group and β-TCP/gelatin/BMSCs were implanted in β-TCP/ge- latin group. Each rabbit received HBO for 14 days after surgery. X-ray, hostologic and mechanical analysis were used to evaluate the repair in each group. Results （1） X-ray showed that 4 weeks after surgery, bone defect area in control group, n-HA/gelatin group, β-TCP/gelatin group had thin cloudy callus shadow, the area of callus shad- ow in n-HA/gelatin group 〉 that in β-TCP/gelatin group 〉 that in control group. After 8 weeks,a small amount of callus formed in control group, a large amount of new bone callus formed in n-HA/gelatin group and β-TCP/gelatin group. After 12 weeks, in control group the repairment of bone defect unfinished; bone defect was basically com- pleted to repair in n-HA/gelatin group and β-TCP/gelatin group with good cortical continuity, recanalizated marrow cavity. （2） Bone callus grey value demonstrated 12 weeks after surgery, bone callus grey value had statistical signifi- cance in control groups, n-HA/gelatin group and β-TCP/gelatin group（P 〈 0. 05）. （3） HE staining indicated the formation of bone trabecula in both n-HA/gelatin group and β-TCP/gelatin group was more than that in control group, and residual material could be found in n-HA/gelatin group and β-TCP/gelatin group 4 weeks after surgery. After 8 weeks, no lamellar bone formed in control group, a large number of lamellar bone formed in group n-HA/ gelatin and β-TCP/gelatin. After 12 weeks, a small amount of lamellar bone formed in control group; a large num- ber of lamellar

关 键 词：海水浸泡 骨缺损 高压氧 骨髓间充质干细胞 n-HA/明胶 β-TCP/明胶 

分 类 号：R683.41[医药卫生—骨科学]