定坤丹对多囊卵巢模型大鼠生殖功能的影响  

Eeffects of Ding Kundan on the reproductive function of polycystic ovary model rats

作　　者：宋玉荣[1] 王文艳[1] 卫兵[1] Song Yurong Wan Wenyan Wei Bing(Dept of Gynecology and Obstetrics, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601)

机构地区：[1]安徽医科大学第二附属医院妇产科,合肥230601

出　　处：《安徽医科大学学报》2016年第10期1473-1477,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金青年科学基金(编号:81100412);安徽省公益性研究联动计划项目(编号:5101ID04041)

摘　　要：目的研究定坤丹对多囊卵巢综合征(PCOS)模型大鼠生殖功能的影响。方法采用丙酸睾酮注射液联合绒促性腺素造模法建立PCOS模型,比较给药前后大鼠卵巢形态学、性激素水平、卵巢中血管内皮生长因子(VEGF)及子宫内膜同源框基因A10(HOXA10)表达情况。结果与模型组大鼠比较,定坤丹组卵巢内黄体数增加,血清促黄体生成素(LH)与雄激素(T)下降,卵泡刺激素(FSH)上升,卵巢VEGF表达下降,子宫HOXA10基因表达上升,差异有统计学意义(P<0.05)。结论定坤丹对PCOS模型大鼠具有促排卵、提高子宫内膜容受性作用。其机制可能与调节多囊卵巢模型大鼠体内性激素水平、减少卵巢中VEGF表达、增加子宫HOXA10表达有关。Objective To study the influence of Ding Kundan on the reproductive function of polycystic ovary syn-drome model rats. Methods The PCOS model was established by using testosterone propionate injection combined with humen chorinic gonadotropin to compare rat ovarian morphology, sex hormones levels, the expression of vascu- lar endothelial growth factor（VEGF） in ovary and homeobox gene A10（HOXAIO） in uterus before and after drug administration. Results In a comparison to the model group, the number of ovarian corpus luteum of the Ding Kun- dan group was increased, and the serum luteinizing hormone （LH） and androgens （T） level of the Ding Kundan group declined, but follicle stimulating hormone（FSH） increased, the expression of VEGF in ovary reduced while HOXAIO in uterine rose. On top of that, their differences were statistically significant（ P 〈 0. 05 ）. Conclusion Ding Kundan has the function of promoting ovulation and can increase the role of endometrial receptivity. The mechanism may relate to the regulation of the serum sex hormones levels, the decrease of VEGF expression in ova- ry, and the addition of HOXAIO in uterus in PCOS model rats.

关 键 词：多囊卵巢综合征 定坤丹 血管内皮生长因子 同源框基因A10 

分 类 号：R711.51[医药卫生—妇产科学]