异源三倍体百合为母本的杂交后代GISH分析  

GISH Analysis of the Progenies from Allotriploid Lily as Female Parent

作　　者：王红[1,2] 高婷婷[1] 辛昊阳 戴兆霞 席梦利[1] WANG Hong GAO Ting-ting XIN Hao-yang DAI Zhao-xia and XI Meng-li(Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China Institute of Vegetable Science, Jiangsu .4 cademy of Agricultural Sciences, Nanjing 21 0014, China)

机构地区：[1]南京林业大学林学院南方现代林业协同创新中心,南京210037 [2]江苏省农业科学院蔬菜研究所,南京210014

出　　处：《园艺学报》2016年第9期1834-1838,共5页Acta Horticulturae Sinica

基　　金：国家林业局‘948’项目(2013-4-34);江苏省农业科技自主创新资金项目[CX(12)2018];江苏高校优势学科建设工程项目

摘　　要：百合异源三倍体是渐渗育种的常用亲本。为了评价异源三倍体‘Royal Lace’的育种价值,采用基因组原位杂交技术分析了‘Royal Lace’及其与亚洲百合‘Pollyanna’的6个杂种后代的基因组组成。研究结果显示：‘Royal Lace’含有24条亚洲百合染色体和12条麝香百合染色体,为异源三倍体,包括1条亚洲百合重组染色体和1条麝香百合重组染色体。6个杂种后代均为非整倍体,染色体数27-32,不同杂种后代的重组染色体及重组位点存在差异,杂种后代基因组组成的不同是后代表型变异的遗传基础,因此,‘Royal Lace’是百合渐渗育种及非整倍性育种的良好亲本。Allotriploid lily is a common parent for introgression breeding, in order to evaluate the breeding value of the allotriploid lily ‘ Royal Lace＇, we analyzed the genome composition of ‘Royal Lace＇ and 6 progenies from‘Royal Lace＇ × ‘Pollyanna＇ using genomic in situ hybridization. The results showed that ‘Royal Lace＇ is allotriploid, which consists of 24 Asiatic chromosomes and 12 Longiflorum chromosomes, including 1 recombinant Asiatic chromosome and 1 recombinant Longiflorum chromosome. The 6 progenies are all aneuploids, the number of chromosomes of the progenies ranges from 27 to 32. There are differences in recombination chromosomes and break points of different progenies. The different genome composition of the progenies is the genetic basis of morphological variations. Therefore, ‘Royal Lace＇ is a promising parent for introgression breeding and aneuploidy breeding in Lilium.

关 键 词：百合 异源三倍体 非整倍体 GISH 

分 类 号：S682.2[农业科学—观赏园艺]