饲喂不同剂量RbCl对棉铃虫的影响以及标记效果  

Effects of feeding RbCl with different dosages on Helicoverpa armigera(Hübner)(Lepidoptera: Noctuidae) and marking efficiency

作　　者：李国平[1] 崔建新[2] 黄建荣[1] 田彩红[1] 封洪强[1] 钟景[1] 黄博[1] 宋国晶[1] LI Guo-ping CUI Jian-xin HUANG Jian- tong TIAN Cai-hong FENG Hong-qiang ZHONG Jing HUANG Bo SONG Guo-jing(Henan Key Laboratory of Crop Pest Control, Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China）.)

机构地区：[1]河南省农作物病虫害防治重点实验室,农业部华北南部作物有害生物综合治理重点实验室,河南省农业科学院植物保护研究所,郑州450002 [2]河南科技学院植保系,河南新乡453003

出　　处：《生态学杂志》2016年第10期2768-2773,共6页Chinese Journal of Ecology

基　　金：转基因重大专项(2014ZX08012-004和2016ZX08012004-007);河南省农业科学院优秀青年科技基金项目(2016YQ15);河南省留学人员科技活动项目资助

摘　　要：微量元素铷(Rb)标记技术花费低、易操作、对环境安全,是研究昆虫种群动态、迁飞、扩散、配偶竞争、寄主选择等常用的方法。标记技术的基本要求是不影响标记对象的寿命和行为。本文通过将不同浓度的氯化铷(Rb Cl)添加到人工饲料中饲喂棉铃虫幼虫,研究了不同浓度的RbCl对棉铃虫的存活、生长发育以及飞行能力的影响和标记效果。结果表明:含有1000~4000 mg·L^(-1)RbCl的人工饲料可以有效地标记棉铃虫,且不影响棉铃虫的生命活动,Rb滞留时间不少于10 d,同时标记的雄虫可以将Rb传递给未标记的雌虫,这为利用此技术研究棉铃虫迁移扩散和交配竞争等奠定了基础。Marking insects with Rb is a low cost, easy to use and environmentally safe technique in entomological research for understanding the insect population dynamics, migration, dispersal, mating competition, feeding behavior, and other ecological interactions. The ideal marker should persist without inhibiting the insect＇ s ＂normal＂ biology. The present study investigated the influence of Rb incorporated into larvae diet on the marking efficiency and life parameters of cotton bollworm, Helicoverpa armigera （Htibner） （Lepidoptera： Noctuidae ）. Feeding 1000-4000 mg ~ L-1 RbC1 in artificial diet effectively marked the adults without any negative effect on the survival, development and flight. Rb could be detected in the adults for at least 10 days. Further- more, Rb was also detected in the unmarked （not fed RbC1） females when they mated with males feeding on a diet containing RbC1. Therefore, 1000-4000 mg · L^-1 are the suitable RbC1 concentrations for marking cotton bollworm.

关 键 词：微量元素 RB 标记技术 棉铃虫 生长发育 

分 类 号：S433[农业科学—农业昆虫与害虫防治]